BACKGROUND: A simple high-performance liquid chromatography (HPLC) method was developed for the determination of albumin in patients' urine samples without coeluting proteins and was compared with the immunoturbidimetric determination of albumin. Urine albumin is important biomarker in diabetic patients, but part of it is immuno-nonreactive. METHODS: Albumin was determined by high-performance liquid chromatography (HPLC), UV detection at 280 nm, Zorbax 300SB-C3 column. Immunoturbidimetric analysis was performed using commercial kit on automatic biochemistry analyzer COBAS INTEGRA® 400, Roche Diagnostics GmbH, Manheim, Germany. RESULTS: The HLPC method was fully validated. No significant interference with other proteins (transferrin, α-1-acid glycoprotein, α-1-antichymotrypsin, antitrypsin, hemopexin) was found. The results from 301 urine samples were compared with immunochemical determination. We found a statistically significant difference between these methods (P = 0.0001, Mann-Whitney test). CONCLUSION: New simple HPLC method was developed for the determination of urine albumin without coeluting proteins. Our data indicate that the HPLC method is highly specific and more sensitive than immunoturbidimetry.

• Klíčová slova
• high-performance liquid chromatography, microalbuminuria, proteinuria, urine albumin,
• MeSH
• albuminurie moč   MeSH
• albuminy analýza   MeSH
• analýza moči MeSH
• časové faktory MeSH
• lidé MeSH
• vysokoúčinná kapalinová chromatografie * MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• albuminy MeSH

OBJECTIVE: Thyroid diseases are among the most common endocrinopathies and metabolic disorders. Hypothyroidism is caused by insufficient production of thyroid hormones with a higher prevalence in women. Causes for the development of endocrine diseases may be mutations in genes that encode peptide hormones. The aim of this scientific study was to determine the genotype and allele frequencies of the rs104893657 variant of the PAX8 gene and to determine the genotype versus phenotype association. METHODS: The study population consisted of 135 women from northeastern Slovakia who were divided on the basis of screening into two groups: a control group without diagnosed hypothyroidism (CG = 67) and a group of women with hypothyroidism (HY = 68). Biochemical markers - thyroid-stimulating hormone (TSH), prealbumin (PREA), calcium (Ca), phosphorus (P), and alkaline phosphatase (ALP) were determined using Cobas Integra 400 plus, Cobas e411 analysers (Roche). Genotyping was performed using TaqMan® SNP Genotyping Assay instrument 7500 Fast Real-Time PCR Systems (Applied Biosystem). RESULTS: Student's t-test revealed a statistically significant difference between CG and HY in biochemical parameters: TSH (p < 0.001), P (p = 0.008). By Chi-square test we found no statistically significant difference in the representation of genotypes (p = 0.788) in the rs104893657 polymorphism of PAX8 gene. The T allele was not associated with hypothyroidism in Slovak women (p = 0.548). In CC genotype we found statistically significant difference between CG and HY in parameters TSH (p < 0.001) and P (p = 0.006). CONCLUSION: The mutant T allele was detected at low frequency in both groups of women studied. The association of the T allele with the development of hypothyroidism in Slovak women was not confirmed. The results of this work provide initial information on the distribution of genotypes and alleles in the studied variant of PAX8 gene in the Slovak female population.

• Klíčová slova
• PAX8 gene, hypothyroidism, marker, phenotype, women,
• MeSH
• genotyp MeSH
• hypotyreóza * epidemiologie   genetika   MeSH
• lidé MeSH
• polymorfismus genetický MeSH
• thyreotropin genetika   MeSH
• transkripční faktor PAX8 genetika   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Slovenská republika epidemiologie   MeSH
• Názvy látek
• PAX8 protein, human MeSH Prohlížeč
• thyreotropin MeSH
• transkripční faktor PAX8 MeSH

OBJECTIVE: The aim of the study was to evaluate the variant (rs2910829) of the PDE4D gene in relation to its influence on biochemical, anthropometric and physiological parameters in patients with coronary artery disease and healthy subjects of the Eastern Slovak population. METHODS: The male group consisted of 72 individuals and the female group consisted of 132 individuals. On the basis of clinical screening the subjects were divided into two groups - with ischaemic heart disease and control group. Genomic DNA was isolated from peripheral blood using a commercial NucleoSpin® Blood Machenery-Nagel kit. Molecular genetic analysis of the polymorphism under study was performed using the StepOne™ Real-Time PCR System instrument. The lipid profile markers TC, HDL, LDL, TG were measured by Cobas Integra 400 plus biochemical analyser, and systolic and diastolic blood pressure using a digital blood pressure monitor. Among anthropometric parameters, body height and weight, waist and hip circumference were measured and BMI and WHR indices were calculated. RESULTS: A statistically significant (p = 0.018) possible association between the mutant T allele and ischaemic heart disease was found in men. In women, we found a statistically significant difference in the systolic (p = 0.013) and diastolic blood parameters (p = 0.005) in the CC genotype. In the group of women, we found statistically significant differences in all observed anthropometric parameters and in LDL and TC markers. In the group of men divided on the basis of BMI, statistical significance was found in systolic blood pressure (p = 0.028). In the group of women with ischaemic heart disease, we found a negative correlation between BMI and HDL. CONCLUSION: The study contributes to new findings of the representation of genotypes and alleles of the rs2910829 PDE4D gene polymorphism in the Slovak population. This is a pilot study. Interactions between genotype and observed anthropometric, physiological and biochemical markers were confirmed.

• Klíčová slova
• BMI, PDE4D gene, cardiovascular disease, diastolic, lipids, systolic,
• MeSH
• antropometrie MeSH
• cyklické nukleotidfosfodiesterasy, typ 4 genetika   MeSH
• index tělesné hmotnosti MeSH
• ischemická choroba srdeční * genetika   MeSH
• lidé MeSH
• nemoci koronárních tepen * MeSH
• pilotní projekty MeSH
• rizikové faktory MeSH
• tělesná výška MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cyklické nukleotidfosfodiesterasy, typ 4 MeSH
• PDE4D protein, human MeSH Prohlížeč

UNLABELLED: Increased concentration of high sensitivity C-reactive protein (hsCRP) is the new predictor of myocardial infarction, brain strokes, damage peripheral veins and sudden death. The aim of the study was to give an overview of current prevalence of individual risky concentrations of hsCRP in the adult population in two regions in the Czech Republic and to find a relationship to the indicators of fat tissue quantity. METHODS: test involved a representative sample of 516 adults aged 18 to 65 years (191 men and 322 women), with permanent residence in the Jablonec nad Nisou a Príbram regions. Period of testing: spring of 2004 and 2005. HsCRP was determined using the latex immunoprecipitation method with turbidimetric measurement on Cobas Integra 400 plus analyzer. The risk of cardiovascular disease (CVD) was set according to the scale derived by the American Heart Association. Body fat was measured with TANITA BF 410 MA, Omron and a calliper on 4 places on the body. Waist circumference was measured half-way between the anthropometric landmarks of the iliocristale and the lower angle of the ribs. Body mass index (BMI) was calculated using Quetelet formula: weight/height2. Statistical processing was done with Statgraphics Plus, version 7.1, using categorical data analysis with the chi2 statistic and Spearman's correlation analysis. RESULTS: 20.2% of cases with high CVD risk were identified in the whole sample of adults. Levels of hsCRP exceeding 10 mg/l (infectious disease indicator) at the time of the test were found in 3.9% cases (5.6% in the Jablonec region, 2.3% in the Príbram region). There were no significant regional differences for individual CVD risk categories; however, there was statistically significant difference between the two regions in terms of average hsCRP levels (with higher levels in the Jablonec region). Gender had a statistically significant impact on hsCRP levels: high CVD risk was recorded in 14.8% men and in 23.3% women. Levels of CRP indicating inflammatory disease were more frequent in women (4.7%) than in men (2.6%). The frequency of risk levels of hsCRP in adults significantly grows with age. Spearman's correlation analysis showed the closest relationship between hsCRP and body fat weight (r(S) = 0.5124). A statistically important positive CRP levels relationship was shown both in subcutaneous fat and visceral fat. CONCLUSION: One fifth of the adult population in two regions of the Czech Republic have at the present time levels of hsCRP which indicate a high cardiovascular risk. The risk is higher in women than in men and it doubles starting at 50 years of age and over. Positive correlation between hsCRP levels and body fat indicators was conformed both for visceral and subcutaneous fat.

• MeSH
• adipozita MeSH
• C-reaktivní protein analýza   MeSH
• dospělí MeSH
• kardiovaskulární nemoci krev   etiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• rizikové faktory MeSH
• senioři MeSH
• složení těla * MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• C-reaktivní protein MeSH