High levels of catecholamines are cardiotoxic and may trigger acute myocardial infarction (AMI). Similarly, the synthetic catecholamine isoprenaline (ISO) evokes a pathological state similar to AMI. During AMI there is a marked increase of free iron and copper which are crucial catalysts of reactive oxygen species formation. Rutin, a natural flavonoid glycoside possessing free radical scavenging and iron/copper chelating activity, may therefore be potentially useful in reduction of catecholamine cardiotoxicity as was previously demonstrated after its long-term peroral administration. Male Wistar:Han rats received rutin (46 or 11.5 mg kg(-1) i.v.) alone or with necrogenic dose of ISO (100 mg kg(-1) s.c.). Haemodynamic parameters were measured 24h after drug application together with analysis of blood, myocardial content of elements and histological examination. Results were confirmed by cytotoxicity studies using cardiomyoblast cell line H9c2. Rutin in a dose of 46 mg kg(-1) aggravated ISO-cardiotoxicity while the dose of 11 mg kg(-1) had no effect. These unexpected results were in agreement with in vitro experiments, where co-incubation with larger concentrations of rutin significantly augmented ISO cytotoxicity. Our results, in contrast to previous studies in the literature, suggest that the reported positive effects of peroral administration of rutin were unlikely to have been mediated by rutin per se but probably by its metabolite(s) or by some other, at this moment, unknown adaptive mechanism(s), which merit further investigation.

• MeSH
• agonisté adrenergních beta-receptorů farmakologie   MeSH
• cévní rezistence účinky léků   MeSH
• chelátory farmakologie   MeSH
• funkční vyšetření srdce MeSH
• glutathion metabolismus   MeSH
• interpretace statistických dat MeSH
• isoprenalin farmakologie   MeSH
• kardiomyocyty účinky léků   metabolismus   patologie   MeSH
• katecholaminy antagonisté a inhibitory   toxicita   MeSH
• krysa rodu Rattus MeSH
• minutový srdeční výdej účinky léků   MeSH
• myokard patologie   MeSH
• nemoci srdce chemicky indukované   patologie   prevence a kontrola   MeSH
• potkani Wistar MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• rutin farmakologie   MeSH
• scavengery volných radikálů metabolismus   MeSH
• velikost orgánu účinky léků   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• agonisté adrenergních beta-receptorů MeSH
• chelátory MeSH
• glutathion MeSH
• isoprenalin MeSH
• katecholaminy MeSH
• reaktivní formy kyslíku MeSH
• rutin MeSH
• scavengery volných radikálů MeSH