The effect of kainic acid, a structural analogue and specific agonist of glutamate, was studied on the Na(+)-dependent binding and uptake of this amino acid in cerebral cortex preparations from 7-day-old and 30-day-old mice. The specific binding of glutamate to a crude synaptic membrane fraction and uptake into cortical slices increased several fold during this period. Kainic acid (0.5 mM or 5 mM) significantly reduced glutamate binding and this effect was more pronounced in membrane fractions from older animals. In contrast to this, the inhibitory action of kainic acid on glutamate uptake was twofold more potent in 7-day-old mice. The results are discussed from the viewpoint of the relationship between the Na(+)-dependent binding of glutamate and its uptake.

• MeSH
• glutamáty metabolismus   MeSH
• kinetika MeSH
• kyselina kainová metabolismus   MeSH
• mozková kůra růst a vývoj   metabolismus   MeSH
• myši MeSH
• sodík metabolismus   MeSH
• techniky in vitro MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• glutamáty MeSH
• kyselina kainová MeSH
• sodík MeSH