This study evaluated the influence of silymarin (SM) and polyphenolic fraction (PF) of silymarin on cholesterol absorption in rats fed on high cholesterol diet (HCD). HCD induced a remarkable increase in hepatic, plasma, VLDL and LDL cholesterol, a decrease in HDL cholesterol and an elevation in triacylglycerol (TAG) levels in plasma, VLDL and in the liver. SM and PF were administered as dietary supplements (1.0%) in HCD for 18 days. Intestinal cholesterol absorption was measured by dual-isotope plasma ratio method, which calculates percent of cholesterol absorption from the ratio of two labelled cholesterol doses, one given intragastrically (14C) and one intravenously (3H). Silymarin and PF significantly reduced cholesterol absorption in rats fed on HCD and caused significant decreases in plasma and VLDL cholesterol and content of cholesterol and TAG in the liver. The level of HDL cholesterol was significantly increased after silymarin, but not after administration of PF. The levels of TAG in plasma and VLDL were not affected by either silymarin or PF. These results suggest that the inhibition of cholesterol absorption caused by silymarin and its polyphenolic fraction could be a mechanism contributing to the positive changes in plasma cholesterol lipoprotein profile and in lipid content in liver.

• MeSH
• antioxidancia farmakologie   MeSH
• azetidiny farmakologie   MeSH
• cholesterol dietní farmakokinetika   MeSH
• cholestyraminová pryskyřice farmakologie   MeSH
• ezetimib MeSH
• fenoly farmakologie   MeSH
• flavonoidy farmakologie   MeSH
• intestinální absorpce účinky léků   MeSH
• játra účinky léků   metabolismus   MeSH
• krysa rodu Rattus MeSH
• lipoproteiny VLDL krev   MeSH
• lipoproteiny metabolismus   MeSH
• polyfenoly MeSH
• silymarin farmakologie   MeSH
• triglyceridy farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• azetidiny MeSH
• cholesterol dietní MeSH
• cholestyraminová pryskyřice MeSH
• ezetimib MeSH
• fenoly MeSH
• flavonoidy MeSH
• lipoproteiny VLDL MeSH
• lipoproteiny MeSH
• polyfenoly MeSH
• silymarin MeSH
• triglyceridy MeSH