1. Hypertension appears to be associated with both abnormalities in ion transport and disturbances in lipid metabolism. The aim of the present study was to search for alterations in erythrocyte ion transport in hereditary hypertriglyceridaemic rats. This strain is characterized by a pronounced elevation of blood pressure which correlates with the plasma triacylglycerol level. 2. Erythrocyte Na+ content was elevated in hypertriglyceridaemic rats due to an increased ouabain-resistant Na+ net uptake. This was caused not only by the acceleration of bumetanide-sensitive Na+ inward co-transport, but also by the augmentation of bumetanide-resistant Na+ inward leak. A moderate enhancement of bumetanide-sensitive Rb+ uptake was seen in hypertriglyceridaemic animals, but there were no changes in bumetanide-resistant Rb+ leak. 3. The plasma triacylglycerol level correlated positively not only with blood pressure (P < 0.001) but also with ouabain-resistant Na+ transport (P < 0.002), bumetanide-sensitive Na+ and K+ (Rb+) co-transport (P < 0.01 and P < 0.001, respectively), bumetanide-resistant Na+ leak (P < 0.02) and erythrocyte Na+ content (P < 0.05). 3. Thus the association of augmented ouabain-resistant Na+ transport with elevated blood pressure in this form of experimental hypertension might be mediated by genetic disorders of lipid metabolism.

• MeSH
• bumetanid farmakologie   MeSH
• draslík metabolismus   MeSH
• erytrocyty účinky léků   metabolismus   MeSH
• hypertriglyceridemie krev   patofyziologie   MeSH
• iontový transport účinky léků   MeSH
• krevní tlak fyziologie   MeSH
• krysa rodu Rattus MeSH
• ouabain farmakologie   MeSH
• potkani Wistar MeSH
• rubidium metabolismus   MeSH
• sodík metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• bumetanid MeSH
• draslík MeSH
• ouabain MeSH
• rubidium MeSH
• sodík MeSH

The possible association of plasma lipids (triglycerides and cholesterol) with erythrocyte Na+ content (Na+i) and/or with alterations in red cell Na+ and K+ (Rb+) transport was studied in a population of F2 hybrids obtained by crossing hypertensive Prague hereditary hypertriglyceridaemic (HTG) rats with normotensive Lewis rats. The obtained data indicated a strong cosegregation (p < 0.001) of plasma triglycerides with erythrocyte Na+ content. This was the cause for the close correlation of plasma triglycerides with the Na(+)-K+ pump activity (measured as ouabain-sensitive Na+ extrusion). On the contrary, there was only marginal association (p < 0.05) of erythrocyte Na+ content with plasma cholesterol which was significantly (p < 0.01) related to burnetanide-sensitive Rb+ uptake mediated by the Na(+)-K+ cotransport system. Na+ leak (bumetanide-resistant net Na+ uptake) correlated positively with blood pressure in female but not in male F2 rats. The close association between plasma triglycerides and erythrocyte Na+ content suggests that ion transport alterations might contribute to mechanisms responsible for the cosegregation of blood pressure with plasma triglycerides in HTG x Lewis F2 hybrids.

• MeSH
• cholesterol krev   MeSH
• erytrocyty metabolismus   MeSH
• inbrední kmeny potkanů MeSH
• křížení genetické MeSH
• krysa rodu Rattus MeSH
• potkani inbrední LEW MeSH
• rubidium krev   MeSH
• sodík-draslík-chloridové symportéry MeSH
• sodík krev   MeSH
• transportní proteiny metabolismus   MeSH
• triglyceridy krev   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• cholesterol MeSH
• rubidium MeSH
• sodík-draslík-chloridové symportéry MeSH
• sodík MeSH
• transportní proteiny MeSH
• triglyceridy MeSH