Freshwater fish Dotaz Zobrazit nápovědu
The paper presents a systematic survey of some cestodes parasitizing freshwater fish in Italy. The following eight species were recorded: Monobothrium wageneri, Cyathocephalus truncatus, Triaenophorus nodulosus (plerocercoids and adults), Bothriocephalus acheilognathi, B. claviceps, Ligula intestinalis (plerocercoids), Schistocephalus sp. (plerocercoids) and Proteocephalus percae from Perca fluviatilis which is reported from freshwater fish in Italy for the first time. All the tapeworms recovered are described and figured.
- MeSH
- Cestoda klasifikace izolace a purifikace ultrastruktura MeSH
- cestodózy parazitologie veterinární MeSH
- druhová specificita MeSH
- nemoci ryb parazitologie MeSH
- ryby parazitologie MeSH
- sladká voda MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Itálie MeSH
UNLABELLED: Currently, new species of freshwater fish trypanosomes, which are economically important parasites, are being described based on subjectively selected features, i.e., their cell morphology and the host species. We have performed detailed phylogenetic and haplotype diversity analyses of all 18S rRNA genes available for freshwater fish trypanosomes, including the newly obtained sequences of Trypanosoma carassii and Trypanosoma danilewskyi. Based on a sequence similarity of 99.5%, we divide these trypanosomes into 15 operational taxonomic units, and propose three nominal scenarios for distinguishing T. carassii and other aquatic trypanosomes. We find evidences for the existence of a low number of freshwater fish trypanosomes, with T. carassii having the widest geographic and host ranges. Our analyses support the existence of an umbrella complex composed of T. carassii and two sister species. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42995-023-00191-0.
- Klíčová slova
- 18S rRNA, Freshwater fish trypanosomes, Morphology, Phylogeny,
- Publikační typ
- časopisecké články MeSH
Bacterial diseases are common in ornamental fish, more frequently associated with ubiquitous bacteria from the aquarium environment. The disease can lead to fish mortality and cause high economic losses if not rapidly controlled. The aim of this study was to identify the main causative bacterial agents of infection in ornamental fish with different clinical signs. A total of 126 freshwater fish, from 12 families and 38 species, with clinical signs were collected in a wholesaler in São Paulo, SP, Brazil. Samples were taken from the eye, skin ulcers, kidneys, and gills, plated on MacConkey, CHROMagar Orientation, and blood agar and incubated under aerobic and anaerobic conditions. Bacterial identification was performed by MALDI-TOF mass spectrometry. From the 126 studied animals, 112 were positive for bacterial isolation. Among the positive animals, 32.1% presented infection caused by a single bacterial species, while in the remaining 67.9%, two to six different bacterial species were identified. A total of 259 bacterial strains were obtained and classified among 46 bacterial species. The species of higher frequency were Aeromonas veronii (26.3%), Aeromonas hydrophilla (16.2%), Shewanella putrefaciens (7.3%), Citrobacter freundii (8.1%), Vibrio albensis (5.8%), and Klebsiella pneumoniae (4.2%). MALDI-TOF MS showed to be a rapid method for diagnosis of bacterial disease outbreaks in ornamental fish establishments.
- Klíčová slova
- Animal health, Bacterial disease, Diagnosis in fish, Fish disease,
- MeSH
- Aeromonas * MeSH
- lidé MeSH
- nemoci ryb * MeSH
- ryby MeSH
- sladká voda MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Brazílie MeSH
Nontuberculous mycobacteria (NTM) are potentially pathogenic agents commonly found in natural ecosystems, while food is considered to be another source of NTM for humans. We investigated a total of 92 tissue samples of freshwater fish and fish products: fish directly obtained from ponds (n=25), retail fresh (n=23) and frozen fish (n=23) and smoked fish products (n=21). Culture examination for the presence of mycobacteria was positive in 11 (11.9%) from all the examined samples. The 15 obtained isolates were identified as Mycobacterium fortuitum (n=5), M. immunogenum (n=2), M. phocaicum/ mucogenicum (n=1), M. neoaurum (n=2), M. peregrinum (n=2), M. porcinum (n=1) and M. senegalense/houstonense/conceptionense (n=2). NTM DNA was found in one (4.0%) sample of fresh fish from ponds and in 60.9% and 91.3% of retail fresh and frozen fish, respectively. None of the smoked fish products contained NTM DNA. The results of our study suggest that freshwater fish and fish products, especially retail frozen fish, might be a reservoir of NTM for humans, and proper handling and treatment before consumption of such products is recommended.
- MeSH
- DNA analýza metabolismus MeSH
- lidé MeSH
- molekulární typizace MeSH
- mražené potraviny analýza ekonomika mikrobiologie MeSH
- Mycobacterium fortuitum klasifikace růst a vývoj izolace a purifikace metabolismus MeSH
- Mycobacterium klasifikace růst a vývoj izolace a purifikace metabolismus MeSH
- mykobakteriózy mikrobiologie MeSH
- potrava z moře (živočišná) analýza ekonomika mikrobiologie MeSH
- potraviny konzervované analýza ekonomika mikrobiologie MeSH
- rybí výrobky analýza ekonomika mikrobiologie MeSH
- ryby metabolismus mikrobiologie MeSH
- sladká voda MeSH
- zdroje nemoci MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA MeSH
A survey of the occurrence of mycobacteria was conducted from 717 freshwater fish (25 species) in two water reservoirs, five ponds and two farms in the Czech Republic. A total of 2182 tissue samples from these fish were examined using the conventional culture method. Thirteen mycobacterial isolates were obtained from 12 (1.7%) fish belonging to nine species. Isolates were identified using sequence analysis of the 16SrRNA gene as: Mycobacterium algericum, M. fortuitum, M. gordonae, M. insubricum, M. kumamotonense, M. nonchromogenicum, two isolates of M. peregrinum, M. terrae and M. triplex. Mycobacteria were isolated more frequently from fish skin and gills than from internal organs or muscles.
- MeSH
- Mycobacterium * genetika izolace a purifikace MeSH
- mykobakteriózy epidemiologie veterinární MeSH
- nemoci ryb epidemiologie mikrobiologie MeSH
- prevalence MeSH
- RNA ribozomální 16S genetika MeSH
- rybníky MeSH
- ryby MeSH
- sladká voda * MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- RNA ribozomální 16S MeSH
Mycobacteriosis in fish is a chronic progressive ubiquitous disease caused by Mycobacterium marinum, M. gordonae and M. fortuitum in most cases. The aim of this study was to describe the morphology and distribution of lesions in 322 freshwater ornamental fish across 36 species. Granulomatous inflammation was diagnosed by gross examination and histopathology testing in 188 fish (58.4%); acid-fast rods (AFR) were determined in only 96 (51.1%) fish from 19 species after Ziehl-Neelsen staining. The most often affected organs with AFR were the kidney (81.2%), digestive tract (54.1%), liver (48.2%), spleen (45.9%) and skin (21.2%); sporadically, AFR were found in the branchiae (9.4%) and gonads (4.7%). In 14 randomly selected fish originating from four different fish tanks, the distribution of mycobacterial infection was studied by culture examination of the skin, gills, muscle tissue, digestive tract, liver, spleen and kidney. In 12 fish, the species M. marinum, M. gordonae, M. fortuitum, M. triviale, and M. avium subsp. hominissuis (serotypes 6 and 8 and genotype IS901- and IS1245+) were detected; mixed infection caused by different mycobacterial species was documented in five of them.
- MeSH
- Mycobacterium izolace a purifikace fyziologie MeSH
- mykobakteriózy mikrobiologie patologie veterinární MeSH
- nemoci ryb mikrobiologie patologie MeSH
- ryby MeSH
- sladká voda * MeSH
- zánět MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- Coccidia růst a vývoj izolace a purifikace MeSH
- Eimeria růst a vývoj izolace a purifikace MeSH
- fluorescence MeSH
- kokcidióza parazitologie veterinární MeSH
- mořská voda MeSH
- nemoci ryb parazitologie MeSH
- Perciformes parazitologie MeSH
- ryby parazitologie MeSH
- sladká voda MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
The sequences of 10 conservative regions (CR) of minicircles of 6 selected isolates of freshwater fish trypanosomes have typical organization of this region with high degree of sequence conservation. The comparison with CRs of other trypanosomatids showed that freshwater fish trypanosomes represent a compact separate group within the genus Trypanosoma. The alignment of all sequences obtained revealed, however, the existence of 2 types of CRs in sequenced minicircles, with the differences concentrated in a short region. Taxonomic consequences of these results are discussed.
- MeSH
- kinetoplastová DNA genetika MeSH
- konzervovaná sekvence genetika MeSH
- molekulární sekvence - údaje MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- ryby parazitologie MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- sladká voda MeSH
- Trypanosoma genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- kinetoplastová DNA MeSH
The parasite communities of the freshwater fish species roach ( Rutilus rutilus), bitterling ( Rhodeus sericeus amarus) and perch ( Perca fluviatilis) were investigated during a major flood. Differences in parasite community structure due to changes in the host environment were expected. We therefore tested for differences in parasite species abundance and diversity as a consequence of the flood. Potential changes in parasite community structure due to seasonal influences were also considered. We found differences in the composition of parasite species and the proportion of ecto- and endoparasites in the three host species. The parasite community of roach was richer in species than those of perch and bitterling. Roach harboured more ectoparasite than endoparasite species. Parasite communities of both perch and bitterling were richer in endoparasite species and had a lower number of ectoparasite species. Parasite communities of the three different host species responded in different ways to environmental change. Considering all parasite species, no effect of either season or flood was found on the species diversity in either perch or bitterling. The flood caused an increase in the parasite species diversity in roach. For roach and perch, higher parasite abundance were found before the flood compared to after flood. However, no difference was found between the different seasons after the flood. By contrast, bitterling had the highest parasite abundance in the season after the flood. When analysing ecto- and endoparasites separately, a decrease in ectoparasite abundance was found in roach and perch, probably related to the flood, and due mainly to a decrease in monogenean species. A significant increase in the abundance of endoparasites was found in perch. In bitterling, the post-flood increase in ectoparasites was due to a high abundance of Gyrodactylus spp. We conclude that the parasite communities of the three fish species responded in different ways to the flood. This may be due to changes in the host life history strategy or in the immune response in conditions of stress.
- MeSH
- Cyprinidae parazitologie MeSH
- ekosystém MeSH
- interakce hostitele a parazita MeSH
- katastrofy MeSH
- okounovití parazitologie MeSH
- paraziti klasifikace izolace a purifikace fyziologie MeSH
- roční období MeSH
- ryby parazitologie MeSH
- sladká voda parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
A redescription of Bothriocephalus acheilognathi a pseudophyllidean cestode parasitizing cyprinid fish is given. The authors outline the hazard of dissemination of this parasite in other Italian fish-producing areas.
- MeSH
- Cestoda izolace a purifikace MeSH
- cestodózy parazitologie veterinární MeSH
- kapři parazitologie MeSH
- nemoci ryb parazitologie MeSH
- sladká voda MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Itálie MeSH