A new microfluidic concept for quantitative whole-column fraction collection of electrophoretically separated zones was developed. The prototype device, fabricated on a polycarbonate disk by injection molding, integrated electrophoretic separation channels with fraction collection reservoirs distributed along the separation channel. The microdevice was designed in a CD-like format to use the centrifugal force for moving the liquid in the microchannels. A serpentine shape of the separation channel was selected to create segments for quantitative whole-column fraction collection. The operation was tested with visual monitoring of isotachophoretic separation and collection of cationic dyes.

• MeSH
• DNA chemie   izolace a purifikace   MeSH
• mikrofluidika přístrojové vybavení   metody   MeSH
• miniaturizace přístrojové vybavení   metody   MeSH
• peptidy chemie   izolace a purifikace   MeSH
• proteiny chemie   izolace a purifikace   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA MeSH
• peptidy MeSH
• proteiny MeSH