Acetylcholine (ACh)-mediated vagal transmission as well as nonneuronal ACh release are considered cardioprotective in pathological situations with increased sympathetic drive such as ischemia-reperfusion and cardiac remodeling. ACh action is terminated by hydrolysis by the cholinesterases (ChEs), acetylcholinesterase, and butyrylcholinesterase. Both ChEs exist in multiple molecular variants either soluble or anchored by specific anchoring proteins like collagen Q (ColQ) anchoring protein and proline-rich membrane anchoring protein (PRiMA). Here we assessed the expression of specific ChE molecular forms in different heart compartments using RT-qPCR. We show that both ChEs are expressed in all heart compartments but display different expression patterns. The acetylcholinesterase-T variant together with PRiMA and ColQ is predominantly expressed in rat atria. Butylcholinesterase is found in all heart compartments and is accompanied by both PRiMA and ColQ anchors. Its expression in the ventricular system suggests involvement in the nonneuronal cholinergic system. Additionally, two PRiMA variants are detected throughout the rat heart.

• Klíčová slova
• acetylcholine, acetylcholinesterase, acétylcholine, acétylcholinestérase, butyrylcholinesterase, butyrylcholinestérase, collagen Q anchoring protein, proline-rich membrane anchoring protein, « collagen Q anchoring protein », « proline-rich membrane anchoring protein »,
• MeSH
• acetylcholin metabolismus   MeSH
• acetylcholinesterasa analýza   metabolismus   MeSH
• butyrylcholinesterasa analýza   metabolismus   MeSH
• GPI-vázané proteiny analýza   metabolismus   MeSH
• izoenzymy analýza   metabolismus   MeSH
• kolagen analýza   metabolismus   MeSH
• krysa rodu Rattus MeSH
• kvantitativní polymerázová řetězová reakce MeSH
• membránové proteiny analýza   metabolismus   MeSH
• myokard enzymologie   MeSH
• potkani Wistar MeSH
• proteiny nervové tkáně analýza   metabolismus   MeSH
• stanovení celkové genové exprese MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• acetylcholin MeSH
• acetylcholinesterasa MeSH
• Ache protein, rat MeSH Prohlížeč
• butyrylcholinesterasa MeSH
• ColQ protein, rat MeSH Prohlížeč
• GPI-vázané proteiny MeSH
• izoenzymy MeSH
• kolagen MeSH
• membránové proteiny MeSH
• PRiMA1 protein, rat MeSH Prohlížeč
• proteiny nervové tkáně MeSH