Studies on structure-activity relationship in the antitumor properties of acridine derivatives
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A series of new 3,6,9-trisubstituted acridine derivatives with fluorine substituents on phenyl ring were synthesized and their interaction with calf thymus DNA was investigated. Analysis using UV-Vis absorbance spectra provided valuable information about the formation of the acridine-DNA complex. In addition, compounds 8b and 8d were found to display an increased binding affinity (K = 2.32 and 2.28 × 106 M-1, respectively). Topo I/II inhibition mode assays were also performed, and the results verify that the novel compounds display topoisomerase I and II inhibitory activity; compounds 8a, 8b and 8c completely inhibited topoisomerase I activity at a concentration of 60 × 10-6 M, but only compound 8d showed partial ability to inhibit topoisomerase II at concentrations of 30 and 50 × 10-6 M. The ability of the derivatives to impair cell proliferation was tested through an analysis of cell cycle distribution, quantification of cell number, viability studies, metabolic activity measurement and clonogenic assay. The content and localization of the derivatives in cells were analyzed using flow cytometry and fluorescence microscopy. The compounds 8b and 8d altered the physiochemical properties and improved antiproliferative activity in A549 human lung carcinoma cells (compound 8d displayed the highest level of activity, 4.25 × 10-6 M, after 48 h).
- Klíčová slova
- 3,6,9-Trisubstituted acridine derivatives, Cytotoxicity, Fluorine, Lung carcinoma cells, Spectroscopic techniques, calf thymus DNA, topoisomerases I/II,
- MeSH
- akridiny chemická syntéza chemie farmakologie MeSH
- buňky A549 MeSH
- DNA-topoisomerasy I metabolismus MeSH
- DNA-topoisomerasy typu II metabolismus MeSH
- DNA účinky léků MeSH
- halogenace MeSH
- inhibitory topoisomerasy I chemická syntéza chemie farmakologie MeSH
- inhibitory topoisomerasy II chemická syntéza chemie farmakologie MeSH
- lidé MeSH
- molekulární struktura MeSH
- proliferace buněk účinky léků MeSH
- protinádorové látky chemická syntéza chemie farmakologie MeSH
- screeningové testy protinádorových léčiv MeSH
- skot MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- vztahy mezi strukturou a aktivitou MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- akridiny MeSH
- calf thymus DNA MeSH Prohlížeč
- DNA-topoisomerasy I MeSH
- DNA-topoisomerasy typu II MeSH
- DNA MeSH
- inhibitory topoisomerasy I MeSH
- inhibitory topoisomerasy II MeSH
- protinádorové látky MeSH
