Histone deacetylase (HDAC) inhibitors have shown beneficial effects in animal models of cardiovascular diseases. We hypothesized that HDAC inhibitor, sodium valproate (VPA), has cardiac and vascular protective effects in rats with pressure overload cardiac hypertrophy induced by transverse aortic constriction (TAC). Sections of the heart were visualized after hematoxylin and eosin staining, picrosirius red staining and immunohistochemistry. The expression of genes related to cardiac hypertrophy, fibrosis, and oxidative stress was determined by quantitative real-time polymerase chain reaction. The aortic ring tension analysis was conducted using both the ascending aorta and descending thoracic aorta. TAC increased the expression of hypertrophic, fibrotic, and oxidative stress genes, which was attenuated by VPA. In the ascending aorta with intact endothelium, there was a significant decrease in the relaxation response, which was recovered by VPA treatment. These results indicate that VPA has cardiac and vascular protective effects in rats with pressure overload cardiac hypertrophy.

• MeSH
• aorta účinky léků   patofyziologie   chirurgie   MeSH
• arteriální tlak účinky léků   MeSH
• fibróza MeSH
• funkce levé komory srdeční účinky léků   MeSH
• hypertrofie levé komory srdeční metabolismus   patologie   patofyziologie   prevence a kontrola   MeSH
• inhibitory histondeacetylas farmakologie   MeSH
• kyselina valproová farmakologie   MeSH
• ligace MeSH
• modely nemocí na zvířatech MeSH
• myokard metabolismus   patologie   MeSH
• oxidační stres účinky léků   MeSH
• potkani Sprague-Dawley MeSH
• regulace genové exprese MeSH
• remodelace komor účinky léků   MeSH
• vazodilatace účinky léků   MeSH
• vazodilatancia farmakologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• inhibitory histondeacetylas MeSH
• kyselina valproová MeSH
• vazodilatancia MeSH

We evaluated the time course of activation of endocardial endothelial cells and cardiomyocytes in adult rats with pressure overload induced by the abdominal aortic constriction. The silver staining technique for nucleolar organizer region was used to mark an increased transcriptional activity of the cells. An increased number of nucleoli was already detected in the endocardial endothelium of the left ventricle 5 h post-operatively, while the response of cardiomyocytes was still absent. Twenty-four h after constriction, the activation of the endocardial endothelium and cardiomyocytes was evident in both ventricles. A similar delay was observed between the nucleolar activation in vascular endothelium and smooth muscle cells of the abdominal aorta. In contrast, these cells of the thoracic aorta did not exhibit any significant increase of the transcriptional activity. The sequential stimulation of the transcriptional activity of the left ventricular endocardial endothelial cells and the subjacent cardiac myocytes due to pressure overload is in accord with the view that the endocardium may serve as a mechanotransducer which converts the hemodynamic changes into the signals that influence the growth of the cardiac myocytes.

• MeSH
• aorta MeSH
• cévní endotel metabolismus   MeSH
• endokard cytologie   metabolismus   MeSH
• genetická transkripce MeSH
• konstrikce MeSH
• krevní tlak * MeSH
• krysa rodu Rattus MeSH
• myokard cytologie   metabolismus   MeSH
• organizátor jadérka metabolismus   MeSH
• potkani Wistar MeSH
• svaly hladké cévní cytologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH