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A method for determining supernatant-free spectra generated from fluorescently stained cells in suspension
J. Plášek, D. Gášková,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- fluorescenční spektrometrie * přístrojové vybavení MeSH
- karbocyaniny chemie MeSH
- membránové potenciály MeSH
- Saccharomyces cerevisiae cytologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Here we present a fluorometric method for direct determination of supernatant-free fluorescence spectra generated from fluorescently stained cells in suspension. The key element in the new technique is the design of an adapter to a standard cuvette holder that makes it possible to measure front-face fluorescence spectra from thin layers of cells spun down to the bottom of a spectrofluorometric cuvette. We have demonstrated the applicability of this approach and its analytical potential using the suspensions of yeast cells stained with the potentiometric dye of 3,3'-dipropylthiadicarbocyanine, diS-C3(3), and with the specific cell-wall marker calcofluor.
Citace poskytuje Crossref.org
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- $a A method for determining supernatant-free spectra generated from fluorescently stained cells in suspension / $c J. Plášek, D. Gášková,
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- $a Here we present a fluorometric method for direct determination of supernatant-free fluorescence spectra generated from fluorescently stained cells in suspension. The key element in the new technique is the design of an adapter to a standard cuvette holder that makes it possible to measure front-face fluorescence spectra from thin layers of cells spun down to the bottom of a spectrofluorometric cuvette. We have demonstrated the applicability of this approach and its analytical potential using the suspensions of yeast cells stained with the potentiometric dye of 3,3'-dipropylthiadicarbocyanine, diS-C3(3), and with the specific cell-wall marker calcofluor.
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