Mango processing generates significant amounts of residues (35-65%) that may represent environmental problems owed to improper disposal. The use of mango byproducts as substrates to produce hyaluronic acid (HA) is an attractive alternative to reduce the cost of substrate. In this study, we evaluated the potential of hydrolyzates from mango peels and seeds to produce HA by Streptococcus equi. subsp. zooepidemicus. The physicochemical characterization of mango residues showed that the seeds contain a higher amount of holocellulose (cellulose and hemicellulose), which amounts 54.2% (w/w) whereas it only represents 15.5% (w/w) in the peels. Mango peels, however, are composed mainly of hot water-extractives (62% w/w, that include sucrose, fructose, glucose and organic acids). A higher concentration of monosaccharides (39.8 g/L) was obtained from the enzymatic hydrolysis (with Macerex) of peels as compared to seeds (24.8 g/L with Celuzyme). From mango peels, hydrolyzates were obtained 0.6 g/L HA, while 0.9 g/L HA were obtained with hydrolyzates from mango seeds. These results demonstrate that mango byproducts have the potential to be used for production of HA.
- MeSH
- celulosa metabolismus MeSH
- fermentace MeSH
- hydrolýza MeSH
- kyselina hyaluronová * biosyntéza metabolismus MeSH
- Mangifera * mikrobiologie chemie MeSH
- monosacharidy metabolismus MeSH
- semena rostlinná chemie mikrobiologie metabolismus MeSH
- Streptococcus equi * metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
Aqueous solutions of ionic liquids (ILs) with surface active properties were used as extraction solvents, taking advantage of their impressive solvation properties, in a green microwave-assisted solid-liquid extraction method (IL-MA-SLE) for the extraction of flavonoids from passion fruit and mango leaves. The extraction method was combined with high-performance liquid chromatography and photodiode-array detection (HPLC-PDA) and optimized by response surface methodology using the Box-Behnken experimental design. Under optimum conditions, the extraction efficiency of six structurally different IL-based surfactants was evaluated. Thus, imidazolium-, guanidinium- and pyridinium-type ILs with different tailorable characteristics, such as side chain length and multicationic core, were assessed. The decylguanidinium chloride ([C10Gu+][Cl-]) IL-based surfactant was selected as key material given its superior performance and its low cytotoxicity, for the determination of flavonoids of several samples of Passiflora sp. and Mangifera sp. leaves from the Canary Islands, and using as target analytes: rutin, quercetin and apigenin. The analysis of 50 mg of plant material only required 525 µL of the low cytotoxic IL-based surfactant solution at 930 mM, 10.5 min of microwave irradiation at 30 °C and 50 W, which involves a simpler, faster, more efficient and greener method in comparison with other strategies reported in the literature for obtaining bioactive compounds profiles from plants.
- MeSH
- flavonoidy chemie izolace a purifikace MeSH
- iontové kapaliny chemie MeSH
- listy rostlin chemie MeSH
- Mangifera chemie MeSH
- mikrovlny MeSH
- Passiflora chemie MeSH
- povrchově aktivní látky chemie MeSH
- rostlinné extrakty chemie MeSH
- rozpouštědla chemie MeSH
- rutin chemie MeSH
- Publikační typ
- časopisecké články MeSH
Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols. Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.). Certain gallotannin preparations promoted mineralization to a greater degree than others. The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization. Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels. Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity. In conclusion, gallotannin incorporation aided mineralization but reduced cytocompatibility.
- MeSH
- alkalická fosfatasa metabolismus MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální polysacharidy MeSH
- biokompatibilní materiály MeSH
- biomimetika metody MeSH
- fosforečnany vápenaté MeSH
- fyziologická kalcifikace účinky léků MeSH
- hydrogely chemie MeSH
- hydrolyzovatelné taniny metabolismus farmakologie MeSH
- lidé MeSH
- Mangifera chemie MeSH
- minerály chemie MeSH
- osteoblasty metabolismus MeSH
- polyfenoly chemie MeSH
- polysacharidy chemie MeSH
- regenerace kostí MeSH
- rostlinné extrakty chemie MeSH
- rostliny metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Species of the Fusarium fujikuroi species complex (FFC) cause a wide spectrum of often devastating diseases on diverse agricultural crops, including coffee, fig, mango, maize, rice, and sugarcane. Although species within the FFC are difficult to distinguish by morphology, and their genes often share 90% sequence similarity, they can differ in host plant specificity and life style. FFC species can also produce structurally diverse secondary metabolites (SMs), including the mycotoxins fumonisins, fusarins, fusaric acid, and beauvericin, and the phytohormones gibberellins, auxins, and cytokinins. The spectrum of SMs produced can differ among closely related species, suggesting that SMs might be determinants of host specificity. To date, genomes of only a limited number of FFC species have been sequenced. Here, we provide draft genome sequences of three more members of the FFC: a single isolate of F. mangiferae, the cause of mango malformation, and two isolates of F. proliferatum, one a pathogen of maize and the other an orchid endophyte. We compared these genomes to publicly available genome sequences of three other FFC species. The comparisons revealed species-specific and isolate-specific differences in the composition and expression (in vitro and in planta) of genes involved in SM production including those for phytohormome biosynthesis. Such differences have the potential to impact host specificity and, as in the case of F. proliferatum, the pathogenic versus endophytic life style.
- MeSH
- fungální proteiny genetika metabolismus MeSH
- Fusarium genetika izolace a purifikace patogenita MeSH
- genom fungální * MeSH
- hostitelská specificita genetika MeSH
- kukuřice setá mikrobiologie MeSH
- Mangifera mikrobiologie MeSH
- metabolom MeSH
- molekulární evoluce MeSH
- Orchidaceae mikrobiologie MeSH
- polymorfismus genetický * MeSH
- Publikační typ
- časopisecké články MeSH
- Klíčová slova
- neuritogen,
- MeSH
- adjuvantní chemoradioterapie škodlivé účinky MeSH
- adjuvantní chemoterapie škodlivé účinky MeSH
- alkylace účinky léků MeSH
- biologická terapie metody využití MeSH
- cytostatické látky * škodlivé účinky toxicita MeSH
- diabetes mellitus 2. typu farmakoterapie MeSH
- dospělí MeSH
- kombinovaná farmakoterapie MeSH
- kurkumin aplikace a dávkování metabolismus terapeutické užití MeSH
- lidé středního věku MeSH
- lidé MeSH
- Mangifera metabolismus MeSH
- metastázy nádorů farmakoterapie MeSH
- mitóza účinky léků MeSH
- nádory prsu farmakoterapie MeSH
- nádory sigmoidea farmakoterapie MeSH
- nemoci nervového systému * chemicky indukované MeSH
- paliativní péče MeSH
- poruchy senzitivity diagnóza farmakoterapie MeSH
- protokoly protinádorové léčby MeSH
- riboflavin aplikace a dávkování metabolismus terapeutické užití MeSH
- somatosenzorické poruchy * diagnóza farmakoterapie MeSH
- stilbeny metabolismus terapeutické užití MeSH
- vitamin B6 aplikace a dávkování metabolismus terapeutické užití MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- bolesti hlavy farmakoterapie MeSH
- farmakopea homeopatická jako téma MeSH
- lidé MeSH
- Mangifera účinky léků MeSH
- psychický stres farmakoterapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- kazuistiky MeSH
