Mismatch repair genes (MMR) play an essential role in DNA repair. MMR mutations predominantly in MLH1, MSH2, MSH6, PMS2, and rarely in PMS1, may cause the production of abnormally short or inactivated proteins. The antiapoptotic protein survivin functions in the inhibition of apoptosis, regulation of cell division and also enhances angiogenesis. Both MMRP and survivin are considered to be powerful prognostic parameters. This study was designed to determine the relationship between MMRP and survivin in colon lesions. The study included 113 cases of colon carcinoma and 51 cases of colon polyps. Survivin expression and MMRP status were assessed by immunohistochemistry. In each section, expression, intensity of immunostaining and percentage of labeled cells were analyzed. In carcinomas, immunoreaction was detected in 100/113 cases for MLH1 (88.5%), 112/113 cases for MSH2 (99.1%), 110/113 cases for MSH6 (97.3%), and 103/113 cases for PMS2 (91.2%). Survivin was shown in 47/113 cases (41.6%). The statistical analysis confirmed a significant correlation between the expression of MMRP and survivin in the assessed parameters. All 51 polyp samples were positive for MLH1, MSH2, MSH6 and PMS2. Only 8 of those (15.7%) were positive for survivin. Statistically significant differences were observed between the expression of MMRP and survivin. In conclusion, this study revealed that MMRP may suppress the antiapoptotic function of survivin through p53 inactivation of its promoter in grade 1 and grade 2 colon carcinomas.
- Klíčová slova
- Colon carcinomas, Colon polyps, Mismatch repair proteins, Survivin,
- MeSH
- adaptorové proteiny signální transdukční metabolismus MeSH
- adenokarcinom enzymologie MeSH
- adenosintrifosfatasy metabolismus MeSH
- DNA vazebné proteiny metabolismus MeSH
- enzymy opravy DNA metabolismus MeSH
- homolog 2 proteinu MutS metabolismus MeSH
- inhibitory apoptózy metabolismus MeSH
- jaderné proteiny metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- mismatch repair endonukleáza PMS2 MeSH
- MutL homolog 1 MeSH
- MutL proteiny MeSH
- nádorové proteiny metabolismus MeSH
- nádory tračníku enzymologie MeSH
- oprava chybného párování bází DNA MeSH
- polypy tlustého střeva enzymologie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- survivin MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adaptorové proteiny signální transdukční MeSH
- adenosintrifosfatasy MeSH
- BIRC5 protein, human MeSH Prohlížeč
- DNA vazebné proteiny MeSH
- enzymy opravy DNA MeSH
- G-T mismatch-binding protein MeSH Prohlížeč
- homolog 2 proteinu MutS MeSH
- inhibitory apoptózy MeSH
- jaderné proteiny MeSH
- mismatch repair endonukleáza PMS2 MeSH
- MLH1 protein, human MeSH Prohlížeč
- MSH2 protein, human MeSH Prohlížeč
- MutL homolog 1 MeSH
- MutL proteiny MeSH
- nádorové proteiny MeSH
- PMS1 protein, human MeSH Prohlížeč
- PMS2 protein, human MeSH Prohlížeč
- survivin MeSH
