Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:oQuantitative reverse transcriptase PCR and methods to examine gene expressionoDifferential display cloningoCloning and library screening by PCRoGenotype and polymorphism analysis of drug and toxicant metabolizing enzymesoBasic, non-PCR based molecular biology methodsPCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

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