V podmienkach in vitro sa sledovali postantibiotický účinok (PAE) a postantibiotický účinok sub-inhibičnej koncentrácie (PA SME) gentamicínu ako aj vplyv týchto farmakodynamických paramet-rov na povrchovú hydrofobicitu troch kmeňov Acinetobacter baumannii. Supresia bakteriálnehorastu (PAE) po krátkodobom účinku gentamicínu (30 min.) v suprainhibičnej koncentrácii 2x MICbola v rozsahu od 0,6 do 3,4 h. Dlhšie PAE sme pozorovali po pôsobení gentamicínu v koncentrácii4x MIC (1,5–5,1 h). Supra-subinhibičné koncentrácie gentamicínu (2x a 4x MIC + 0,2x MIC)spôsobili ešte výraznejšie oneskorenie obnovenia bakteriálneho rastu (PA SME) ako suprainhibičnékoncentrácie u jedného kmeňa (9,1 h a 9,6 h) a v prípade dvoch ďalších kmeňov boli tak účinné, žebakteriálny rast sa neobnovil do 24 h. Povrchová hydrofobicita kmeňov po účinku gentamicínuhodnotená adherenciou baktérií na xylén sa pohybovala v rozsahu od 90,0 % do 99,7 % kontrolnýchhodnôt (bez antibiotika).

The postantibiotic effect (PAE) and postantibiotic effect of subinhibitory concentration (PA SME) ofgentamicin as well as influence of these pharmacodynamic parameters on surface hydrophobicityof three Acinetobacter baumannii strains were studied in vitro. Suppression of bacterial growth(PAE) after a short time exposure of bacteria to gentamicin (30 min) at suprainhibitory concentra -tion 2x MIC was in the range of 0.6 h to 3.4 h. Longer PAE was observed after treatment of bacteriawith gentamicin at 4x MIC (1.5 h – 5.1 h). Supra-subinhibitory concentrations of gentamicin (2x or4x MIC + 0.2x MIC) caused still more effective delay of bacterial regrowth (PA SME) in comparisonwith suprainhibitory concentrations for one strain (9.1 h and 9.6 h) and in the case of further twostrains they were so efficient that regrowth of bacteria was not observed for 24 h. Surfacehydrophobicity of the gentamicin treated strains evaluated by adherence of bacteria to xylene rangedfrom 90.0% to 99.7% of the control values (without antibiotic).

Ústav preventívnej a klinickej medicíny Bratislava

Pharmacodynamic parameters of gentamicin and their influence on surface hydrophobicity of Acinetobacter baumannii

Farmakodynamické parametre gentamicínu a ich vplyv na povrchovú hydrofobicitu Acinetobacter baumannii = Pharmacodynamic parameters of gentamicin and their influence on surface hydrophobicity of Acinetobacter baumannii /

Pharmacodynamic parameters of gentamicin and their influence on surface hydrophobicity of Acinetobacter baumannii /

Lit: 30

Souhrn: eng