-
Je něco špatně v tomto záznamu ?
Ordered expression of virulence genes in Salmonella enterica serovar typhimurium
Kateřina Papežová, Daniela Gregová, J. Jonuschies, Ivan Rychlík
Jazyk angličtina Země Česko
- MeSH
- exprese genu fyziologie genetika imunologie MeSH
- finanční podpora výzkumu jako téma MeSH
- financování vládou MeSH
- genetické techniky trendy využití MeSH
- luciferasy genetika MeSH
- polymerázová řetězová reakce s reverzní transkripcí využití MeSH
- promotorové oblasti (genetika) imunologie účinky léků MeSH
- reportérové geny genetika MeSH
- Salmonella typhimurium genetika imunologie metabolismus MeSH
- transkripční faktory Sp genetika MeSH
- virulence genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
Using transcriptional promoter fusions, we investigated the expression of selected SPI-1 and SPI-2 genes of Salmonella enterica serovar Typhimurium (S. Typhimurium). Promoters of genes related to the invasion of the epithelial cell (hilA, hilC, hilD, invF, sicA, sopA, sopB and sopE2) were active in... ( view more ) Luria-Bertani (LB) medium and LB with butyrate but were suppressed by bile salts and in glucose minimal (M9) medium. Genes related to S. Typhimurium intracellular survival (phoP, ssrA, ssaB, ssaG, sifA, sifB and pipB) were characterized by their expression in stationary phase in LB and M9 medium. Activity of phoP and ssrA promoters indicated that these might be expressed inside the gut. SPI-1 genes were expressed on the transition to stationary phase while SPI-2 genes were expressed in stationary phase. Among SPI-1 genes, those with regulatory functions preceded in expression the effector genes and sop genes were expressed in the order of sopA, sopB and sopE2, showing hierarchy in the expression of S. Typhimurium virulence genes.
Grant č. 1/2422 VEGA -- Grant č. 178 VTP
Bibliografie atd.Lit.: 46
- 000
- 03248naa 2200481 a 4500
- 001
- bmc07502278
- 003
- CZ-PrNML
- 005
- 20111210121404.0
- 008
- 080229s2007 xr e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Papežová, Kateřina $7 xx0136676
- 245 10
- $a Ordered expression of virulence genes in Salmonella enterica serovar typhimurium / $c Kateřina Papežová, Daniela Gregová, J. Jonuschies, Ivan Rychlík
- 314 __
- $a Veterinary Research Institute, Brno
- 500 __
- $a Grant č. 1/2422 VEGA -- Grant č. 178 VTP
- 504 __
- $a Lit.: 46
- 520 9_
- $a Using transcriptional promoter fusions, we investigated the expression of selected SPI-1 and SPI-2 genes of Salmonella enterica serovar Typhimurium (S. Typhimurium). Promoters of genes related to the invasion of the epithelial cell (hilA, hilC, hilD, invF, sicA, sopA, sopB and sopE2) were active in... ( view more ) Luria-Bertani (LB) medium and LB with butyrate but were suppressed by bile salts and in glucose minimal (M9) medium. Genes related to S. Typhimurium intracellular survival (phoP, ssrA, ssaB, ssaG, sifA, sifB and pipB) were characterized by their expression in stationary phase in LB and M9 medium. Activity of phoP and ssrA promoters indicated that these might be expressed inside the gut. SPI-1 genes were expressed on the transition to stationary phase while SPI-2 genes were expressed in stationary phase. Among SPI-1 genes, those with regulatory functions preceded in expression the effector genes and sop genes were expressed in the order of sopA, sopB and sopE2, showing hierarchy in the expression of S. Typhimurium virulence genes.
- 650 _2
- $a exprese genu $x fyziologie $x genetika $x imunologie $7 D015870
- 650 _2
- $a virulence $x genetika $7 D014774
- 650 _2
- $a transkripční faktory Sp $x genetika $7 D051703
- 650 _2
- $a Salmonella typhimurium $x genetika $x imunologie $x metabolismus $7 D012486
- 650 _2
- $a reportérové geny $x genetika $7 D017930
- 650 _2
- $a luciferasy $x genetika $7 D008156
- 650 _2
- $a promotorové oblasti (genetika) $x imunologie $x účinky léků $7 D011401
- 650 _2
- $a polymerázová řetězová reakce s reverzní transkripcí $x využití $7 D020133
- 650 _2
- $a genetické techniky $x trendy $x využití $7 D005821
- 650 _2
- $a finanční podpora výzkumu jako téma $7 D012109
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a financování vládou $7 D005380
- 700 1_
- $a Gregorová, Daniela, $d 1977- $7 xx0037984
- 700 1_
- $a Jonuschies, J.
- 700 1_
- $a Rychlík, Ivan, $d 1966- $7 xx0037983
- 773 0_
- $w MED00011005 $t Folia microbiologica $g Roč. 52, č. 2 (2007), s. 107-114 $x 0015-5632
- 910 __
- $a ABA008 $b A 2403 $c 561 $y 1
- 990 __
- $a 20080229114826 $b ABA008
- 991 __
- $a 20080325131849 $b ABA008
- 999 __
- $a ok $b bmc $g 617898 $s 470330
- BAS __
- $a 3
- BMC __
- $a 2007 $b 52 $c 2 $d 107-114 $i 0015-5632 $m Folia microbiologica $x MED00011005
- LZP __
- $a 2007-22/mkal