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Glucosylglycerate is an osmotic solute and an extracellular metabolite produced by Streptomyces caelestis
S. Pospíšil, P. Halada, M. Petříček, P. Sedmera
Language English Country Czech Republic
- MeSH
- Extracellular Fluid microbiology MeSH
- Research Support as Topic MeSH
- Spectrometry, Mass, Electrospray Ionization methods utilization MeSH
- Glyceric Acids isolation & purification MeSH
- Streptomyces chemistry MeSH
- Trehalose analogs & derivatives isolation & purification MeSH
Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3% NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract--(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium.
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Lit.: 19
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- 024 __
- $a 10.1007/bf02932103 $2 doi
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- 314 __
- $a Institute of Microbiology, Academy of Sciences of the Czech Republic
- 504 __
- $a Lit.: 19
- 520 9_
- $a Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3% NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract--(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium.
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