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Glucosylglycerate is an osmotic solute and an extracellular metabolite produced by Streptomyces caelestis

S. Pospíšil, P. Halada, M. Petříček, P. Sedmera

. 2007 ; 52 (5) : 451-456.

Jazyk angličtina Země Česko

Perzistentní odkaz   https://www.medvik.cz/link/bmc07504114

Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3% NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract--(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium.

Citace poskytuje Crossref.org

Bibliografie atd.

Lit.: 19

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