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Phytase from antarctic yeast strain Cryptococcus laurentii AL27
Pavlova K, Gargova S, Hristozova T, Tankova Z.
Language English Country Czech Republic
- MeSH
- 6-Phytase genetics chemistry metabolism MeSH
- Cryptococcus enzymology genetics isolation & purification metabolism MeSH
- Fungal Proteins genetics chemistry metabolism MeSH
- Kinetics MeSH
- Soil Microbiology MeSH
- Enzyme Stability MeSH
- Temperature MeSH
- Geographicals
- Antarctic Regions MeSH
Cryptococcus laurentii strain AL(27) demonstrating significant potential for intracellular phytase production was selected by 2-step screening of Antarctic yeasts. The strain showed increased phytase activity in a culture medium with 40 g/L sucrose, KH(2)PO(4) providing 5 mg/L phosphorus, and cultivation temperature of 24 degrees C, which relates it to psychrotrophic microorganisms. The enzyme kinetic characteristics according to sodium phytate were K (m) = 0.98 mmol/L, v (lim) = 33.3 mumol g(-1) min(-1). The enzyme had maximum activity at 40 degrees C and acted within a wide pH range: from 2.0 to 5.5, which is of positive significance for its direct inclusion into the feed of monogastric animals.
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Lit.: 28
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- $a 10.1007/s12223-008-0004-3 $2 doi
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- $a Pavlova, K.
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- $a Laboratory of Applied Microbiology, Institute of Microbiology, Bulgarian Academy of Science, Plovdiv
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- $a Lit.: 28
- 520 9_
- $a Cryptococcus laurentii strain AL(27) demonstrating significant potential for intracellular phytase production was selected by 2-step screening of Antarctic yeasts. The strain showed increased phytase activity in a culture medium with 40 g/L sucrose, KH(2)PO(4) providing 5 mg/L phosphorus, and cultivation temperature of 24 degrees C, which relates it to psychrotrophic microorganisms. The enzyme kinetic characteristics according to sodium phytate were K (m) = 0.98 mmol/L, v (lim) = 33.3 mumol g(-1) min(-1). The enzyme had maximum activity at 40 degrees C and acted within a wide pH range: from 2.0 to 5.5, which is of positive significance for its direct inclusion into the feed of monogastric animals.
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- $a 6-fytasa $x genetika $x chemie $x metabolismus $7 D010832
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- $a Cryptococcus $x enzymologie $x genetika $x izolace a purifikace $x metabolismus $7 D003454
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