-
Je něco špatně v tomto záznamu ?
Validated HPLC-MS-MS method for determination of azithromycin in human plasma
Barrett B, Borek-Dohalský V, Fejt P, Vaingátová S, Huclová J, Nemec B, Jelínek I.
Jazyk angličtina Země Německo
Typ dokumentu validační studie
NLK
ProQuest Central
od 2001-01-01 do 2010-12-31
Medline Complete (EBSCOhost)
od 2003-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2001-01-01 do 2010-12-31
- MeSH
- antibakteriální látky chemie krev MeSH
- azithromycin krev MeSH
- časové faktory MeSH
- EDTA krev MeSH
- hmotnostní spektrometrie metody MeSH
- lidé MeSH
- referenční hodnoty MeSH
- reprodukovatelnost výsledků MeSH
- roxithromycin chemie MeSH
- senzitivita a specificita MeSH
- ultrafiltrace MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- validační studie MeSH
A validated, highly sensitive, and selective HPLC method with MS-MS detection has been developed for quantitative determination of azithromycin (AZI) in human Na2EDTA plasma. Roxithromycin (ROX) was used as internal standard. Human plasma containing AZI and internal standard was ultrafiltered through Centrifree Micropartition devices and the concentration of AZI was determined by isocratic HPLC-MS-MS. Multiple reaction monitoring mode (MRM) was used for MS-MS detection. The calibration plot was linear in the concentration range 2.55-551.43 ng mL(-1). Inter-day and Intra-day precision and accuracy of the proposed method were characterized by R.S.D and percentage deviation, respectively; both were less than 8%. Limit of quantification was 2.55 ng mL(-1). The proposed method was used to determine the pharmacokinetic profile of AZI (250-mg tablets).
Citace poskytuje Crossref.org
- 000
- 00000naa 2200000 a 4500
- 001
- bmc07520349
- 003
- CZ-PrNML
- 005
- 20241121122817.0
- 008
- 090331s2005 gw e eng||
- 009
- AR
- 024 __
- $a 10.1007/s00216-005-0018-5 $2 doi
- 035 __
- $a (PubMed)16091946
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Barrett, Blanka $7 xx0325887
- 245 10
- $a Validated HPLC-MS-MS method for determination of azithromycin in human plasma / $c Barrett B, Borek-Dohalský V, Fejt P, Vaingátová S, Huclová J, Nemec B, Jelínek I.
- 314 __
- $a Bioanalytical Laboratory, Cepha s.r.o., 32300, Plzen, Czech Republic
- 520 9_
- $a A validated, highly sensitive, and selective HPLC method with MS-MS detection has been developed for quantitative determination of azithromycin (AZI) in human Na2EDTA plasma. Roxithromycin (ROX) was used as internal standard. Human plasma containing AZI and internal standard was ultrafiltered through Centrifree Micropartition devices and the concentration of AZI was determined by isocratic HPLC-MS-MS. Multiple reaction monitoring mode (MRM) was used for MS-MS detection. The calibration plot was linear in the concentration range 2.55-551.43 ng mL(-1). Inter-day and Intra-day precision and accuracy of the proposed method were characterized by R.S.D and percentage deviation, respectively; both were less than 8%. Limit of quantification was 2.55 ng mL(-1). The proposed method was used to determine the pharmacokinetic profile of AZI (250-mg tablets).
- 650 _2
- $a antibakteriální látky $x chemie $x krev $7 D000900
- 650 _2
- $a azithromycin $x krev $7 D017963
- 650 _2
- $a vysokoúčinná kapalinová chromatografie $x metody $7 D002851
- 650 _2
- $a EDTA $x krev $7 D004492
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a hmotnostní spektrometrie $x metody $7 D013058
- 650 _2
- $a referenční hodnoty $7 D012016
- 650 _2
- $a reprodukovatelnost výsledků $7 D015203
- 650 _2
- $a roxithromycin $x chemie $7 D015575
- 650 _2
- $a senzitivita a specificita $7 D012680
- 650 _2
- $a časové faktory $7 D013997
- 650 _2
- $a ultrafiltrace $7 D014462
- 655 _2
- $a validační studie $7 D023361
- 700 1_
- $a Bořek-Dohalský, Václav
- 700 1_
- $a Fejt, Petr
- 700 1_
- $a Vaingátová, Silvie $7 xx0129032
- 700 1_
- $a Huclová, Jitka $7 xx0036626
- 700 1_
- $a Němec, Bohdan $7 xx0128808
- 700 1_
- $a Jelínek, Ivan, $d 1960- $7 jo2004232786
- 773 0_
- $w MED00006638 $t Analytical and bioanalytical chemistry $g Roč. 383, č. 2 (2005), s. 210-217 $x 1618-2642
- 910 __
- $a ABA008 $b x $y 9 $z 0
- 990 __
- $a 20090310084605 $b ABA008
- 991 __
- $a 20241121122811 $b ABA008
- 999 __
- $a ok $b bmc $g 638152 $s 490951
- BAS __
- $a 3
- BMC __
- $a 2005 $b 383 $c 2 $d 210-217 $i 1618-2642 $m Analytical and bioanalytical chemistry $x MED00006638
- LZP __
- $a 2009-B2/ipme
