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Genotypic characterisation of vancomycin-resistant Enterococcus faecium isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic
Kolar M, Pantucek R, Vagnerova I, Kesselova M, Sauer P, Matouskova I, Doskar J, Koukalova D, Hejnar P
Language English Country France
Grant support
1A8258
MZ0
CEP Register
Digital library NLK
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NLK
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from 1997 to 2 years ago
Medline Complete (EBSCOhost)
from 2000-01-01 to 2014-12-31
Wiley Online Library (archiv)
from 1997-01-01 to 2012-12-31
- MeSH
- Enterococcus faecalis isolation & purification classification MeSH
- Enterococcus faecium genetics isolation & purification classification MeSH
- Financing, Organized MeSH
- Gram-Positive Bacterial Infections microbiology MeSH
- Hematologic Neoplasms MeSH
- Cross Infection microbiology MeSH
- Humans MeSH
- Neoplasms MeSH
- Cancer Care Facilities MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Vancomycin Resistance genetics MeSH
- Check Tag
- Humans MeSH
- Geographicals
- Czech Republic MeSH
This study describes the first molecular characterisation of clinical isolates of vancomycin-resistant enterococci (VRE) in the Czech Republic. Of 2647 patient isolates of Enterococcus spp. from 1997-2002, 121 (4.6%) were identified as VRE. The most common isolates were VanA+ Enterococcus faecium (78%) and VanB+ Enterococcus faecalis (10%). In addition, five VanA+ E. faecium isolates were obtained from environmental and staff sampling. Macrorestriction analysis of SmaI restriction fragment length polymorphism was performed for 54 VanA+ E. faecium clinical isolates and the five VanA+ E. faecium environmental isolates. Thirty-two unique restriction endonuclease patterns were identified, including two predominant clonal types represented by five or more isolates. Two environmental VanA+ E. faecium isolates were closely related to two patient isolates, which had an identical SmaI macrorestriction pattern. The results indicated potential survival of strains in the hospital environment and possible subsequent transmission to hospitalised patients.
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- $a Genotypic characterisation of vancomycin-resistant Enterococcus faecium isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic / $c Kolar M, Pantucek R, Vagnerova I, Kesselova M, Sauer P, Matouskova I, Doskar J, Koukalova D, Hejnar P
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- $a Department of Microbiology, Faculty of Medicine, Palacky University, Olomouc, Czech Republic. kolar@fnol.cz
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- $a This study describes the first molecular characterisation of clinical isolates of vancomycin-resistant enterococci (VRE) in the Czech Republic. Of 2647 patient isolates of Enterococcus spp. from 1997-2002, 121 (4.6%) were identified as VRE. The most common isolates were VanA+ Enterococcus faecium (78%) and VanB+ Enterococcus faecalis (10%). In addition, five VanA+ E. faecium isolates were obtained from environmental and staff sampling. Macrorestriction analysis of SmaI restriction fragment length polymorphism was performed for 54 VanA+ E. faecium clinical isolates and the five VanA+ E. faecium environmental isolates. Thirty-two unique restriction endonuclease patterns were identified, including two predominant clonal types represented by five or more isolates. Two environmental VanA+ E. faecium isolates were closely related to two patient isolates, which had an identical SmaI macrorestriction pattern. The results indicated potential survival of strains in the hospital environment and possible subsequent transmission to hospitalised patients.
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