Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Leptin affects proliferation-, apoptosis- and protein kinase A-related peptides in human ovarian granulosa cells

Alexander V. Sirotkin, Miloš Mlynček, Alexander V. Makarevich, I. Florkovičová, Ladislav Hetényi

. 2008 ; 57 (3) : 437-442.

Jazyk angličtina Země Česko

Perzistentní odkaz   https://www.medvik.cz/link/bmc07520711

The aim of our in vitro studies was to understand the role of leptin in controlling proliferation, apoptosis, and protein kinase A (PKA) in human ovarian cells. We analyzed the in vitro effects of leptin (0, 1, 10 or 100 ng/ml) on the accumulation of proliferation-related peptides (PCNA, cyclin B1), apoptosis-associated peptide (Bax) and the intracellular signaling molecule PKA in cultured human granulosa cells using immunocytochemistry and Western immunoblotting. It was observed that leptin stimulated in a dose-dependent manner the accumulation of PCNA (at doses 1-100 ng/ml), cyclin B1 (at doses 10 or 100 ng/ml), Bax (at doses 10 or 100 ng/ml) and PKA (at doses 1-100 ng/ml) in cultured human ovarian cells. These observations suggest the ability of leptin to control directly human ovarian cell functions: proliferation, apoptosis, and intracellular messenger PKA.

Citace poskytuje Crossref.org

Bibliografie atd.

Lit.: 19

000      
00000naa 2200000 a 4500
001      
bmc07520711
003      
CZ-PrNML
005      
20111210131220.0
008      
090406s2008 xr e eng||
009      
AR
024    7_
$a 10.33549/physiolres.931090 $2 doi
040    __
$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Sirotkin, Alexander V., $d 1954- $7 xx0116769
245    10
$a Leptin affects proliferation-, apoptosis- and protein kinase A-related peptides in human ovarian granulosa cells / $c Alexander V. Sirotkin, Miloš Mlynček, Alexander V. Makarevich, I. Florkovičová, Ladislav Hetényi
314    __
$a Research Institute of Animal Production, Nitra
504    __
$a Lit.: 19
520    9_
$a The aim of our in vitro studies was to understand the role of leptin in controlling proliferation, apoptosis, and protein kinase A (PKA) in human ovarian cells. We analyzed the in vitro effects of leptin (0, 1, 10 or 100 ng/ml) on the accumulation of proliferation-related peptides (PCNA, cyclin B1), apoptosis-associated peptide (Bax) and the intracellular signaling molecule PKA in cultured human granulosa cells using immunocytochemistry and Western immunoblotting. It was observed that leptin stimulated in a dose-dependent manner the accumulation of PCNA (at doses 1-100 ng/ml), cyclin B1 (at doses 10 or 100 ng/ml), Bax (at doses 10 or 100 ng/ml) and PKA (at doses 1-100 ng/ml) in cultured human ovarian cells. These observations suggest the ability of leptin to control directly human ovarian cell functions: proliferation, apoptosis, and intracellular messenger PKA.
650    _2
$a dospělí $7 D000328
650    _2
$a apoptóza $7 D017209
650    _2
$a western blotting $7 D015153
650    _2
$a buněčný cyklus $7 D002453
650    _2
$a proliferace buněk $7 D049109
650    _2
$a kultivované buňky $7 D002478
650    _2
$a proteinkinasy závislé na cyklickém AMP $x metabolismus $7 D017868
650    _2
$a cyklin B $x metabolismus $7 D019926
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a folikulární buňky $x enzymologie $x imunologie $x patologie $7 D006107
650    _2
$a lidé $7 D006801
650    _2
$a imunohistochemie $7 D007150
650    _2
$a leptin $x metabolismus $7 D020738
650    _2
$a proliferační antigen buněčného jádra $x metabolismus $7 D018809
650    _2
$a rekombinantní proteiny $x metabolismus $7 D011994
650    _2
$a signální transdukce $7 D015398
650    _2
$a protein X asociovaný s bcl-2 $x metabolismus $7 D051028
650    _2
$a financování organizované $7 D005381
700    1_
$a Mlynček, Miloš
700    1_
$a Makarevič, Alexander, $d 1963- $7 xx0157427
700    1_
$a Konýčková, Iveta $7 xx0112808
700    1_
$a Hetényi, Ladislav, $d 1951- $7 uzp2006347003
773    0_
$w MED00003824 $t Physiological research $g Roč. 57, č. 3 (2008), s. 437-442 $x 0862-8408
856    41
$u http://www.biomed.cas.cz/physiolres/pdf/57/57_437.pdf $y plný text volně přístupný
910    __
$a ABA008 $b A 4120 $c 266 $y 9
990    __
$a 20090403092005 $b ABA008
991    __
$a 20090728103534 $b ABA008
999    __
$a ok $b bmc $g 638516 $s 491330
BAS    __
$a 3
BMC    __
$a 2008 $b 57 $c 3 $d 437-442 $i 0862-8408 $m Physiological research $x MED00003824
LZP    __
$a 2009-13/mkme

Najít záznam

Citační ukazatele

Možnosti archivace