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Cellular uptake of phosphorothioate oligonucleotide facilitated by cationic porphyrin: a microfluorescence study
Kocisová E, Praus P, Mojzes P, Sureau F, Stepánek J, Seksek O, Turpin PY
Jazyk angličtina Země Spojené státy americké
NLK
Wiley Online Library (archiv)
od 1996-01-01 do 2012-12-31
Wiley Online Library (archiv)
od 1996-01-01 do 2012-12-31
PubMed
16506169
DOI
10.1002/bip.20495
Knihovny.cz E-zdroje
- MeSH
- antisense oligonukleotidy chemie metabolismus MeSH
- buňky 3T3 MeSH
- financování organizované MeSH
- fluorescenční spektrometrie metody MeSH
- myši MeSH
- porfyriny metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
Cationic 5,10,15,20-tetrakis (1-methyl-4-pyridyl) porphyrin was tested as a delivery agent for oligonucleotides. By using fluorescence microimaging, it has been shown that complexation of the porphyrin to the phosphorothioate analog of dT(15) labeled by rhodamine enabled its nonendocytic penetration into the cell and regular distribution in the cytoplasm and preferentially into the nucleus. Time-resolved microfluorescence spectroscopy revealed that the oligonucleotide integrity was kept. A small fraction of the porphyrin molecules seems to undergo change of the binding mode after internalization, probably due to duplex formation between the oligonucleotide and its cellular target sequences, or due to dissociation of the porphyrin from the oligonucleotide and subsequent interactions in the cellular environment. (c) 2006 Wiley Periodicals, Inc.
Citace poskytuje Crossref.org
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