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The Na+,K+/H+ -antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae
Kinclova-Zimmermannova O, Gaskova D, Sychrova H.
Language English Country Netherlands
NLK
ProQuest Central
from 2003-03-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2006-01-01 to 2014-12-31
Health & Medicine (ProQuest)
from 2003-03-01 to 1 year ago
Wiley Online Library (archiv)
from 2001-01-01 to 2012-12-31
Oxford Journals Open Access Collection
from 2001-04-01
- MeSH
- Potassium metabolism MeSH
- Financing, Organized MeSH
- Hydrogen-Ion Concentration MeSH
- Membrane Potentials MeSH
- Membrane Proteins physiology MeSH
- Sodium-Hydrogen Exchangers physiology MeSH
- Cation Transport Proteins physiology MeSH
- Saccharomyces cerevisiae Proteins physiology MeSH
- Saccharomyces cerevisiae physiology growth & development MeSH
- Sodium metabolism MeSH
There are three different sodium transport systems (Ena1-4p, Nha1p, Nhx1p) in Saccharomyces cerevisiae. The effect of their absence on the tolerance to alkali-metal cations and on the membrane potential was studied. All three sodium transporters were found to participate in the maintenance of Na+, Li+, K+ and Cs+ homeostasis. Measurements of the distribution of a fluorescent potentiometric probe (diS-C3(3) assay) in cell suspensions showed that the lack of all three transporters depolarizes the plasma membrane. The overexpression of the Na+,K+/H+ antiporter Nha1 resulted in the hyperpolarization of the plasma membrane and consequently increased the sensitivity to Cs+, Tl+ and hygromycin B. This is the first evidence that the activity of a Na+,K+/H+ antiporter could play a role in the homeostatic regulation of the plasma membrane potential in yeast cells.
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- $a Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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- $a There are three different sodium transport systems (Ena1-4p, Nha1p, Nhx1p) in Saccharomyces cerevisiae. The effect of their absence on the tolerance to alkali-metal cations and on the membrane potential was studied. All three sodium transporters were found to participate in the maintenance of Na+, Li+, K+ and Cs+ homeostasis. Measurements of the distribution of a fluorescent potentiometric probe (diS-C3(3) assay) in cell suspensions showed that the lack of all three transporters depolarizes the plasma membrane. The overexpression of the Na+,K+/H+ antiporter Nha1 resulted in the hyperpolarization of the plasma membrane and consequently increased the sensitivity to Cs+, Tl+ and hygromycin B. This is the first evidence that the activity of a Na+,K+/H+ antiporter could play a role in the homeostatic regulation of the plasma membrane potential in yeast cells.
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