-
Something wrong with this record ?
Localization of human coagulation factor VIII (hFVIII) in transgenic rabbit by FISH-TSA: identification of transgene copy number and transmission to the next generation
Vladimír Krylov, Tereza Tlapáková, Jaroslav Mácha, Jozef Curlej, L. Ryban, Peter Chrenek
Language English Country Czech Republic
NLK
Free Medical Journals
from 2000
Freely Accessible Science Journals
from 2000
ProQuest Central
from 2005-01-01
Health & Medicine (ProQuest)
from 2005-01-01
ROAD: Directory of Open Access Scholarly Resources
from 2000
- MeSH
- Factor VIII genetics metabolism MeSH
- Animals, Genetically Modified MeSH
- Gene Dosage MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- Rabbits MeSH
- Humans MeSH
- Chromosome Mapping methods MeSH
- Chromosomes, Mammalian MeSH
- Nucleic Acid Amplification Techniques MeSH
- Transgenes genetics MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
For chromosomal localization of the hFVIII human transgene in F2 and F3 generation of transgenic rabbits, FISH-TSA was applied. A short cDNA probe (1250 bp) targeted chromosomes 3, 7, 8, 9 and 18 of an F2 male (animal 1-3-8). Two transgenic offspring (F3) revealed signal positions in chromosome 3 and chromosomes 3 and 7, respectively. Sequencing and structure analysis of the rabbit orthologous gene revealed high similarity to its human counterpart. Part of the sequenced cDNA (1310 bp) served as a probe for FISH-TSA analysis. The rabbit gene was localized in the q arm terminus of the X chromosome. This result is in agreement with reciprocal chromosome painting between the rabbit and the human. The presented FISH-TSA method provides strong signals without any interspecies reactivity.
Lit.: 19
- 000
- 00000naa 2200000 a 4500
- 001
- bmc07523115
- 003
- CZ-PrNML
- 005
- 20111210134638.0
- 008
- 090513s2008 xr e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Krylov, Vladimír $7 xx0128644
- 245 10
- $a Localization of human coagulation factor VIII (hFVIII) in transgenic rabbit by FISH-TSA: identification of transgene copy number and transmission to the next generation / $c Vladimír Krylov, Tereza Tlapáková, Jaroslav Mácha, Jozef Curlej, L. Ryban, Peter Chrenek
- 314 __
- $a Charles University in Prague, Faculty of Science, Department of Cell Biology, Prague
- 504 __
- $a Lit.: 19
- 520 9_
- $a For chromosomal localization of the hFVIII human transgene in F2 and F3 generation of transgenic rabbits, FISH-TSA was applied. A short cDNA probe (1250 bp) targeted chromosomes 3, 7, 8, 9 and 18 of an F2 male (animal 1-3-8). Two transgenic offspring (F3) revealed signal positions in chromosome 3 and chromosomes 3 and 7, respectively. Sequencing and structure analysis of the rabbit orthologous gene revealed high similarity to its human counterpart. Part of the sequenced cDNA (1310 bp) served as a probe for FISH-TSA analysis. The rabbit gene was localized in the q arm terminus of the X chromosome. This result is in agreement with reciprocal chromosome painting between the rabbit and the human. The presented FISH-TSA method provides strong signals without any interspecies reactivity.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a geneticky modifikovaná zvířata $7 D030801
- 650 _2
- $a faktor VIII $x genetika $x metabolismus $7 D005169
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a genová dávka $7 D018628
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a hybridizace in situ fluorescenční $x metody $7 D017404
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a techniky amplifikace nukleových kyselin $7 D021141
- 650 _2
- $a králíci $7 D011817
- 650 _2
- $a transgeny $x genetika $7 D019076
- 650 _2
- $a mapování chromozomů $x metody $7 D002874
- 650 _2
- $a savčí chromozomy $7 D033481
- 700 1_
- $a Tlapáková, Tereza $7 xx0128846
- 700 1_
- $a Mácha, Jaroslav $7 xx0128797
- 700 1_
- $a Čurlej, Jozef $7 xx0151306
- 700 1_
- $a Ryban, L.
- 700 1_
- $a Chrenek, Peter. $7 _BN005620
- 773 0_
- $w MED00011004 $t Folia biologica $g Roč. 54, č. 4 (2008), s. 121-124 $x 0015-5500
- 856 41
- $u https://fb.cuni.cz/Data/files/folia_biologica/volume_54_2008_4/FB2008A0020.pdf $y plný text volně přístupný
- 910 __
- $a ABA008 $b A 970 $c 89 $y 9
- 990 __
- $a 20090513150919 $b ABA008
- 991 __
- $a 20090514102347 $b ABA008
- 999 __
- $a ok $b bmc $g 651203 $s 504375
- BAS __
- $a 3
- BMC __
- $a 2008 $b 54 $c 4 $d 121-124 $i 0015-5500 $m Folia biologica (Praha) $x MED00011004
- LZP __
- $a 2009-18/mkme
