-
Something wrong with this record ?
Functionalization of PVC membrane with ss oligonucleotides for a potentiometric biosensor
Shishkanova TV, Volf R, Krondak M, Král V
Language English Country Great Britain
Document type Evaluation Study
NLK
ScienceDirect (archiv)
from 1993-01-01 to 2009-12-31
- MeSH
- Equipment Failure Analysis MeSH
- Coated Materials, Biocompatible chemistry MeSH
- Biosensing Techniques methods instrumentation MeSH
- Equipment Design MeSH
- DNA Probes genetics chemistry MeSH
- Financing, Organized MeSH
- In Situ Hybridization methods instrumentation MeSH
- Oligonucleotides analysis genetics chemistry MeSH
- Polyvinyl Chloride chemistry MeSH
- Reproducibility of Results MeSH
- Sensitivity and Specificity MeSH
- Publication type
- Evaluation Study MeSH
A novel application of a single stranded (ss) oligonucleotide as an active component of polymeric membrane in an ion-selective electrode (ISE) is described. The original oligonucleotides, oligo(dA)(15), modified by cholesterol, triphenylmethyl and hexadecyl derivatives, were immobilized into poly(vinyl chloride) (PVC) membrane using extraction protocol. In parallel, the adsorption protocol was used to immobilize unmodified oligo(dA)(15) on the PVC membrane based on tridodecylmethyammonium chloride (TDDMA(+)Cl(-)). Immobilization of ss oligonucleotide probe through spacer was more effective for the potentiometric detection of the hybridization between complementary oligonucleotides. It was found that cholesterol-oligo(dA)(15) modified membranes were sensitive toward complementary oligo(dT)(15) in the concentration range 2-80 nM at pH 7. An explanation for the detection mechanism is proposed.
- 000
- 00000naa 2200000 a 4500
- 001
- bmc07526843
- 003
- CZ-PrNML
- 005
- 20111210143459.0
- 008
- 090802s2007 xxk e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Šiškanova, Tat'jana Viktorovna, $d 1964- $7 xx0134892
- 245 10
- $a Functionalization of PVC membrane with ss oligonucleotides for a potentiometric biosensor / $c Shishkanova TV, Volf R, Krondak M, Král V
- 314 __
- $a Department of Analytical Chemistry, Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech Republic. tatiana.shishkanova@vscht.cz <tatiana.shishkanova@vscht.cz>
- 520 9_
- $a A novel application of a single stranded (ss) oligonucleotide as an active component of polymeric membrane in an ion-selective electrode (ISE) is described. The original oligonucleotides, oligo(dA)(15), modified by cholesterol, triphenylmethyl and hexadecyl derivatives, were immobilized into poly(vinyl chloride) (PVC) membrane using extraction protocol. In parallel, the adsorption protocol was used to immobilize unmodified oligo(dA)(15) on the PVC membrane based on tridodecylmethyammonium chloride (TDDMA(+)Cl(-)). Immobilization of ss oligonucleotide probe through spacer was more effective for the potentiometric detection of the hybridization between complementary oligonucleotides. It was found that cholesterol-oligo(dA)(15) modified membranes were sensitive toward complementary oligo(dT)(15) in the concentration range 2-80 nM at pH 7. An explanation for the detection mechanism is proposed.
- 650 _2
- $a financování organizované $7 D005381
- 650 _2
- $a biosenzitivní techniky $x metody $x přístrojové vybavení $7 D015374
- 650 _2
- $a biokompatibilní potahované materiály $x chemie $7 D020099
- 650 _2
- $a DNA sondy $x genetika $x chemie $7 D015342
- 650 _2
- $a design vybavení $7 D004867
- 650 _2
- $a analýza selhání vybavení $7 D019544
- 650 _2
- $a hybridizace in situ $x metody $x přístrojové vybavení $7 D017403
- 650 _2
- $a oligonukleotidy $x analýza $x genetika $x chemie $7 D009841
- 650 _2
- $a polyvinylchlorid $x chemie $7 D011143
- 650 _2
- $a reprodukovatelnost výsledků $7 D015203
- 650 _2
- $a senzitivita a specificita $7 D012680
- 655 _2
- $a hodnotící studie $7 D023362
- 700 1_
- $a Volf, Radko. $7 _AN058645
- 700 1_
- $a Kronďák, Martin, $d 1978- $7 xx0106801
- 700 1_
- $a Král, Vladimír, $d 1949-2019 $7 jo20010088887
- 773 0_
- $w MED00006627 $t Biosensors & bioelectronics $g Roč. 22, č. 11 (2007), s. 2712-2717 $x 0956-5663
- 910 __
- $a ABA008 $b x $y 7
- 990 __
- $a 20090726182400 $b ABA008
- 991 __
- $a 20090924152012 $b ABA008
- 999 __
- $a ok $b bmc $g 670859 $s 530008
- BAS __
- $a 3
- BMC __
- $a 2007 $b 22 $c 11 $d 2712-2717 $i 0956-5663 $m Biosensors & bioelectronics $x MED00006627
- LZP __
- $a 2009-B3/vtme
