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Proteomics analysis of the Francisella tularensis LVS response to iron restriction: induction of the F. tularensis pathogenicity island proteins IglABC
Lenco J, Hubálek M, Larsson P, Fucíková A, Brychta M, Macela A, Stulík J
Jazyk angličtina Země Velká Británie
NLK
ProQuest Central
od 1996-01-01 do 2012-12-31
Medline Complete (EBSCOhost)
od 2006-01-01 do 2014-12-15
Health & Medicine (ProQuest)
od 1996-01-01 do 2012-12-31
Wiley Online Library (archiv)
od 1997-01-01 do 2012-12-31
Public Health Database (ProQuest)
od 1996-01-01 do 2012-12-31
- MeSH
- 2D gelová elektroforéza MeSH
- bakteriální proteiny genetika metabolismus MeSH
- faktory virulence genetika metabolismus MeSH
- financování organizované MeSH
- Francisella tularensis metabolismus patogenita růst a vývoj MeSH
- genomové ostrovy MeSH
- hmotnostní spektrometrie MeSH
- operon MeSH
- proteomika MeSH
- regulace genové exprese u bakterií MeSH
- stanovení celkové genové exprese MeSH
- železo metabolismus MeSH
Francisella tularensis is a highly virulent, facultative intracellular pathogen that causes tularemia in humans and animals. Although it is one of the most infectious bacterial pathogens, little is known about its virulence mechanisms. In this study, the response of F. tularensis live vaccine strain to iron depletion, which simulates the environment within the host, was investigated. In order to detect alterations in protein synthesis, metabolic labeling, followed by 2D-PAGE analysis was used. Globally, 141 protein spots were detected whose levels were significantly altered in the iron-restricted medium. About 65% of the spots were successfully identified using mass spectrometric approaches. Importantly, among the proteins produced at an increased level during iron-limited growth, three proteins were found encoded by the igl operon, located in the F. tularensis pathogenicity island I (FPI). Of these, the IglC and IglA proteins were previously reported to be necessary for full virulence of F. tularensis. These results, obtained at the proteome level, support and confirm recently published data showing that the igl operon genes are transcribed in response to iron limitation
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- $a Proteomics analysis of the Francisella tularensis LVS response to iron restriction: induction of the F. tularensis pathogenicity island proteins IglABC / $c Lenco J, Hubálek M, Larsson P, Fucíková A, Brychta M, Macela A, Stulík J
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- $a Francisella tularensis is a highly virulent, facultative intracellular pathogen that causes tularemia in humans and animals. Although it is one of the most infectious bacterial pathogens, little is known about its virulence mechanisms. In this study, the response of F. tularensis live vaccine strain to iron depletion, which simulates the environment within the host, was investigated. In order to detect alterations in protein synthesis, metabolic labeling, followed by 2D-PAGE analysis was used. Globally, 141 protein spots were detected whose levels were significantly altered in the iron-restricted medium. About 65% of the spots were successfully identified using mass spectrometric approaches. Importantly, among the proteins produced at an increased level during iron-limited growth, three proteins were found encoded by the igl operon, located in the F. tularensis pathogenicity island I (FPI). Of these, the IglC and IglA proteins were previously reported to be necessary for full virulence of F. tularensis. These results, obtained at the proteome level, support and confirm recently published data showing that the igl operon genes are transcribed in response to iron limitation
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