-
Je něco špatně v tomto záznamu ?
The optimization of sample treatment for spectral karyotyping with applications for human tumour cells
T. Loja, P. Kuglik, A. Oltova, P. Smuharova, K. Zitterbart, V. Bajciova, R. Veselska
Jazyk angličtina Země Švýcarsko
Grantová podpora
NR9125
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Zdroj
NLK
Karger Journals
od 2002 do 2009
ProQuest Central
od 2001 do 2015-11-30
Medline Complete (EBSCOhost)
od 2002-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2001 do 2015-11-30
- MeSH
- dítě MeSH
- financování organizované MeSH
- hybridizace nukleových kyselin MeSH
- indoly MeSH
- krevní buňky cytologie účinky léků MeSH
- lidé MeSH
- metafáze účinky léků MeSH
- neuroblastom genetika patologie MeSH
- odběr biologického vzorku metody MeSH
- proteasy farmakologie MeSH
- spektrální karyotypizace metody MeSH
- stárnutí buněk účinky léků MeSH
- Wilmsův nádor genetika patologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
Spectral karyotyping (SKY) represents an important tool for the investigation of the complex chromosomal rearrangements (CCRs) in many human malignancies which may be difficult to characterize by conventional banding techniques. The main goal of our work was to optimize the most important steps in the preparation of molecular cytogenetic slides for a SKY protocol. This approach consisted of optimization of both the aging procedure and protease pretreatment of the slides, with special regard given to the preservation of chromosome structure and shape, as well as to the intensity of hybridization signals. The best results were obtained with a chemical aging procedure using SSC or ethanol in combination with trypsin pretreatment applied at a higher concentration for a shorter period of pretreatment. A resulting protocol for SKY also applicable to human solid tumour cells was subsequently proposed. The practical potential of the SKY technique was demonstrated on examples of two types of human embryonal tumours--neuroblastoma and Wilms' tumour, in which some kinds of chromosomal aberrations were not detectable by means of classic cytogenetic methods. Copyright 2007 S. Karger AG, Basel.
Department of Biology School of Medicine Masaryk University Brno Czech Republic
Department of Medical Genetics University Hospital Brno Brno Czech Republic
Department of Pediatric Oncology University Hospital Brno Brno Czech Republic
- 000
- 00000naa 2200000 a 4500
- 001
- bmc09003997
- 003
- CZ-PrNML
- 005
- 20140716093921.0
- 008
- 091126s2007 sz e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Loja, Tomáš $7 xx0112989 $u Laboratory of Tumor Biology and Genetics, Department of Genetics and Molecular Biology, Institute of Experimental Biology, School of Science, Masaryk University, Brno, Czech Republic; Department of Biology, School of Medicine, Masaryk University, Brno, Czech Republic
- 245 14
- $a The optimization of sample treatment for spectral karyotyping with applications for human tumour cells / $c T. Loja, P. Kuglik, A. Oltova, P. Smuharova, K. Zitterbart, V. Bajciova, R. Veselska
- 314 __
- $a Laboratory of Tumor Biology and Genetics, Department of Genetics and Molecular Biology, Institute of Experimental Biology, School of Science, Masaryk University, Brno, Czech Republic
- 520 9_
- $a Spectral karyotyping (SKY) represents an important tool for the investigation of the complex chromosomal rearrangements (CCRs) in many human malignancies which may be difficult to characterize by conventional banding techniques. The main goal of our work was to optimize the most important steps in the preparation of molecular cytogenetic slides for a SKY protocol. This approach consisted of optimization of both the aging procedure and protease pretreatment of the slides, with special regard given to the preservation of chromosome structure and shape, as well as to the intensity of hybridization signals. The best results were obtained with a chemical aging procedure using SSC or ethanol in combination with trypsin pretreatment applied at a higher concentration for a shorter period of pretreatment. A resulting protocol for SKY also applicable to human solid tumour cells was subsequently proposed. The practical potential of the SKY technique was demonstrated on examples of two types of human embryonal tumours--neuroblastoma and Wilms' tumour, in which some kinds of chromosomal aberrations were not detectable by means of classic cytogenetic methods. Copyright 2007 S. Karger AG, Basel.
- 650 _2
- $a financování organizované $7 D005381
- 650 _2
- $a krevní buňky $x cytologie $x účinky léků $7 D001773
- 650 _2
- $a stárnutí buněk $x účinky léků $7 D016922
- 650 _2
- $a dítě $7 D002648
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a indoly $7 D007211
- 650 _2
- $a metafáze $x účinky léků $7 D008677
- 650 _2
- $a neuroblastom $x genetika $x patologie $7 D009447
- 650 _2
- $a hybridizace nukleových kyselin $7 D009693
- 650 _2
- $a proteasy $x farmakologie $7 D010447
- 650 _2
- $a odběr biologického vzorku $x metody $7 D013048
- 650 _2
- $a spektrální karyotypizace $x metody $7 D032681
- 650 _2
- $a Wilmsův nádor $x genetika $x patologie $7 D009396
- 700 1_
- $a Kuglík, Petr, $d 1957- $7 ola2003204793 $u Laboratory of Tumor Biology and Genetics, Department of Genetics and Molecular Biology, Institute of Experimental Biology, School of Science, Masaryk University, Brno, Czech Republic
- 700 1_
- $a Oltová, Alexandra $7 xx0110415 $u Department of Medical Genetics, University Hospital Brno, Brno, Czech Republic
- 700 1_
- $a Šmuhařová, Petra $7 xx0109643 $u Department of Medical Genetics, University Hospital Brno, Brno, Czech Republic
- 700 1_
- $a Zitterbart, Karel $7 xx0142630 $u Department of Pediatric Oncology, University Hospital Brno, Brno, Czech Republic
- 700 1_
- $a Bajčiová, Viera $7 xx0089689 $u Department of Pediatric Oncology, University Hospital Brno, Brno, Czech Republic
- 700 1_
- $a Veselská, Renata, $d 1968- $7 mzk2003196540 $u Laboratory of Tumor Biology and Genetics, Department of Genetics and Molecular Biology, Institute of Experimental Biology, School of Science, Masaryk University, Brno, Czech Republic
- 773 0_
- $w MED00006332 $t Cytogenetic and genome research $g Roč. 116, č. 3 (2007), s. 186-193 $x 1424-8581
- 910 __
- $a ABA008 $b x $y 8 $z 0
- 990 __
- $a 20091123115031 $b ABA008
- 991 __
- $a 20140716094221 $b ABA008
- 999 __
- $a ok $b bmc $g 699815 $s 562227
- BAS __
- $a 3
- BMC __
- $a 2007 $b 116 $c 3 $d 186-193 $i 1424-8581 $m Cytogenetic and genome research $x MED00006332
- GRA __
- $a NR9125 $p MZ0
- LZP __
- $a 2009-B3/dkme
