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Electron microscopy in situ hybridization: tracking of DNA and RNA sequences at high resolution

Cmarko D, Koberna K.

. 2007 ; 369 () : 213-228.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu srovnávací studie

Perzistentní odkaz   https://www.medvik.cz/link/bmc10000916

Electron microscopy in situ hybridization (EM-ISH) represents a powerful method that enables the localization of specific sequences of nucleic acids at high resolution. We provide here an overview of three different nonisotopic EM-ISH approaches that allow the visualization of nucleic acid sequences in cells. A comparison of various methods with respect to their sensitivity and the structural preservation of the sample is presented, with the aim of helping the reader to choose a convenient hybridization procedure. The post-embedding EM-ISH protocol that currently represents the most widely used technique is described in detail, with a special emphasis on the organization of the cell nucleus.

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