-
Je něco špatně v tomto záznamu ?
Off-targeting and other non-specific effects of RNAi experiments in mammalian cells
Svoboda P.
Jazyk angličtina Země Velká Británie
Typ dokumentu přehledy
- MeSH
- biologické modely MeSH
- financování organizované MeSH
- interferony genetika imunologie metabolismus MeSH
- lidé MeSH
- malá interferující RNA genetika MeSH
- RNA interference MeSH
- signální transdukce fyziologie genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
The RNA interference (RNAi) approach is an efficient and widely used method for silencing plant and animal genes. However, numerous studies have highlighted several obstacles that need to be overcome in order to achieve a specific and efficient silencing effect. Among the major problems of using RNAi in mammalian systems are non-specific effects, such as an undesirable activation of the IFN response, and off-target effects, whereby partial complementarity of an RNAi trigger to non-targeted transcripts causes unwanted silencing effects. The causes of non-specific effects and possible approaches to minimize them are discussed in this review.
- 000
- 00000naa 2200000 a 4500
- 001
- bmc10000980
- 003
- CZ-PrNML
- 005
- 20121122112041.0
- 008
- 100115s2007 xxk e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Svoboda, Petr $7 xx0140547
- 245 10
- $a Off-targeting and other non-specific effects of RNAi experiments in mammalian cells / $c Svoboda P.
- 314 __
- $a Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic. svobodap@img.cas.cz
- 520 9_
- $a The RNA interference (RNAi) approach is an efficient and widely used method for silencing plant and animal genes. However, numerous studies have highlighted several obstacles that need to be overcome in order to achieve a specific and efficient silencing effect. Among the major problems of using RNAi in mammalian systems are non-specific effects, such as an undesirable activation of the IFN response, and off-target effects, whereby partial complementarity of an RNAi trigger to non-targeted transcripts causes unwanted silencing effects. The causes of non-specific effects and possible approaches to minimize them are discussed in this review.
- 650 _2
- $a financování organizované $7 D005381
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a interferony $x genetika $x imunologie $x metabolismus $7 D007372
- 650 _2
- $a biologické modely $7 D008954
- 650 _2
- $a RNA interference $7 D034622
- 650 _2
- $a malá interferující RNA $x genetika $7 D034741
- 650 _2
- $a signální transdukce $x fyziologie $x genetika $7 D015398
- 655 _2
- $a přehledy $7 D016454
- 773 0_
- $w MED00173598 $t Current opinion in molecular therapeutics $g Roč. 9, č. 3 (2007), s. 248-257 $x 1464-8431
- 910 __
- $a ABA008 $b x $y 8
- 990 __
- $a 20100114162108 $b ABA008
- 991 __
- $a 20121122112104 $b ABA008
- 999 __
- $a ok $b bmc $g 703708 $s 566149
- BAS __
- $a 3
- BMC __
- $a 2007 $b 9 $c 3 $d 248-257 $m Current opinion in molecular therapeutics $x MED00173598
- LZP __
- $a 2010-b1/dkme
