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Využití moderních reverzních stacionárních fází na bázi oxidu zirkoničitého pro analýzu bioaktivních peptidů
[Application of modern reversed phases based on zirconium dioxide for the analysis of bioactive peptides]
L. Janečková, J. Sobotníková, E. Tesařová, Z. Bosáková
Jazyk čeština Země Česko
- MeSH
- financování organizované MeSH
- oxidy chemie MeSH
- peptidy analýza MeSH
- vysokoúčinná kapalinová chromatografie přístrojové vybavení MeSH
- zirkonium chemie MeSH
Separation of biologically active peptides was performed on polybutadiene (PBD) and polystyrene (PS) reversed phases based on zirconium dioxide. ZrO2 as an alternative carrier to silicagel offers ion-exchange interactions, which are useful for separation of ionizable compounds. The parameters like buffer concentration and pH, the amount of organic modifier and temperature affected separation of nonapeptides. The retention characteristics (retention factor, resolution, peak symmetry, separation efficiency) were investigated. The systems consisting of acetonitrile and phosphate buffer of basic pH were found suitable for separation of vasopressin-related peptides.
Application of modern reversed phases based on zirconium dioxide for the analysis of bioactive peptides
Lit.: 39
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- $a Lit.: 39
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- $a Separation of biologically active peptides was performed on polybutadiene (PBD) and polystyrene (PS) reversed phases based on zirconium dioxide. ZrO2 as an alternative carrier to silicagel offers ion-exchange interactions, which are useful for separation of ionizable compounds. The parameters like buffer concentration and pH, the amount of organic modifier and temperature affected separation of nonapeptides. The retention characteristics (retention factor, resolution, peak symmetry, separation efficiency) were investigated. The systems consisting of acetonitrile and phosphate buffer of basic pH were found suitable for separation of vasopressin-related peptides.
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