Accumulating evidence suggests that manganese oxide nanoparticles (NPs) show multiple enzyme-mimicking antioxidant activities, which supports their potential in redox-targeting therapeutic strategies for diseases with impaired redox signaling. However, the systemic administration of any NP requires thorough hemocompatibility testing. In this study, we assessed the hemocompatibility of synthesized Mn3O4 NPs, identifying their ability to induce spontaneous hemolysis and eryptosis or impair osmotic fragility. Concentrations of up to 20 mg/L were found to be safe for erythrocytes. Eryptosis assays were shown to be more sensitive than hemolysis and osmotic fragility as markers of hemocompatibility for Mn3O4 NP testing. Flow cytometry- and confocal microscopy-based studies revealed that eryptosis induced by Mn3O4 NPs was accompanied by Ca2+ overload, altered redox homeostasis verified by enhanced intracellular reactive oxygen species (ROS) and reactive nitrogen species (RNS), and a decrease in the lipid order of cell membranes. Furthermore, Mn3O4 NP-induced eryptosis was calpain- and caspase-dependent.
- MeSH
- buněčná membrána * metabolismus účinky léků MeSH
- eryptóza * účinky léků MeSH
- erytrocyty účinky léků metabolismus MeSH
- hemolýza účinky léků MeSH
- kalpain * metabolismus MeSH
- kaspasy * metabolismus MeSH
- lidé MeSH
- nanočástice * chemie MeSH
- oxidy * farmakologie chemie MeSH
- reaktivní formy dusíku * metabolismus MeSH
- reaktivní formy kyslíku * metabolismus MeSH
- sloučeniny manganu * farmakologie chemie MeSH
- vápník * metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
In the current epoch, noble metals/metal oxides with precise structures are needed to develop sustainable products to improve the welfare of human beings and the environment. Nanomaterials in the regime 1 -100 nm scale are a promising material for the research fraternities owing to their stupendous properties. The metallic/metal oxide nanoparticles (silver, gold, copper oxide, iron oxide, magnesium oxide) are gaining significant momentum and need to be extensively studied. Magnesium oxide nanoparticles (MgONPs) are a periclase, white hygroscopic material consisting of Mg2+ ions and O-2 ions in lattice arranged. These nanoparticles can be fabricated through physical, chemical and biological methods. The development of green synthesized MgONPs needs to be ascertained and explored its ultimate in medicine, health, cosmetics, environmental protection, chemical industries, and energy. Therefore, the present review manifests the green synthetic approaches of MgONPs and their impact on crystalline structure and shape. Further, we have provided the antibacterial and anticancer activities of MgONPs thoroughly reported in various kinds of literature. Overall, the unique MgONPs can be plausibly used as safe biomaterials in biomedical applications.
- MeSH
- antibakteriální látky farmakologie chemie MeSH
- kovové nanočástice * chemie MeSH
- lidé MeSH
- nanostruktury * MeSH
- oxid hořečnatý farmakologie chemie MeSH
- oxidy chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Manganese oxides are considered an essential component of natural geochemical barriers due to their redox and sorptive reactivity towards essential and potentially toxic trace elements. Despite the perception that they are in a relatively stable phase, microorganisms can actively alter the prevailing conditions in their microenvironment and initiate the dissolution of minerals, a process that is governed by various direct (enzymatic) or indirect mechanisms. Microorganisms are also capable of precipitating the bioavailable manganese ions via redox transformations into biogenic minerals, including manganese oxides (e.g., low-crystalline birnessite) or oxalates. Microbially mediated transformation influences the (bio)geochemistry of manganese and also the environmental chemistry of elements intimately associated with its oxides. Therefore, the biodeterioration of manganese-bearing phases and the subsequent biologically induced precipitation of new biogenic minerals may inevitably and severely impact the environment. This review highlights and discusses the role of microbially induced or catalyzed processes that affect the transformation of manganese oxides in the environment as relevant to the function of geochemical barriers.
- MeSH
- mangan * chemie MeSH
- minerály chemie MeSH
- oxidace-redukce MeSH
- oxidy * chemie MeSH
- sloučeniny manganu chemie MeSH
- životní prostředí MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Synthetic food colorants are extensively used across the globe regardless of the fact that they induce deleterious side effects when used in higher amounts. In this work, a novel electrochemical sensor based on nickel nanoparticles doped lettuce-like Co3O4 anchored graphene oxide (GO) nanosheets was developed for effective detection of sulfonated azo dye sunset yellow widely used as a food colorant. Hydrothermal synthesis was adopted for the preparation of lettuce-like spinel Co3O4 nanoparticles and Ni-Co3O4 NPs/GO nanocomposite was prepared using ecofriendly and economical sonochemical method. The prepared ternary nanocomposite meticulously fabricated on a screen-printed carbon electrode exhibited remarkable electrocatalytic activity towards sunset yellow determination. This is apparent from the resultant well-defined and intense redox peak currents of Ni-Co3O4 NPs/GO nanocomposite modified electrode at very low potentials. The developed sunset yellow sensor exhibited a high sensitivity of 4.16 μA μM-1 cm-2 and a nanomolar detection limit of 0.9 nM in the linear range 0.125-108.5 μM. Furthermore, experiments were conducted to affirm excellent stability, reproducibility, repeatability, and selectivity of proposed sensor. The practicality of sunset yellow determination using the developed sensor was analyzed in different varieties of food samples including jelly, soft drink, ice cream, and candy resulting in recovery in the range of 96.16%-102.56%.
- MeSH
- azosloučeniny analýza MeSH
- elektrochemické techniky metody MeSH
- grafit MeSH
- kobalt chemie MeSH
- kovové nanočástice chemie MeSH
- limita detekce MeSH
- lineární modely MeSH
- nanokompozity chemie MeSH
- nikl chemie MeSH
- oxid hlinitý chemie MeSH
- oxid hořečnatý chemie MeSH
- oxidy chemie MeSH
- potravinářská barviva analýza MeSH
- reprodukovatelnost výsledků MeSH
- Publikační typ
- časopisecké články MeSH
Inhibition of the human O-linked β-N-acetylglucosaminidase (hOGA, GH84) enzyme is pharmacologically relevant in several diseases such as neurodegenerative and cardiovascular disorders, type 2 diabetes, and cancer. Human lysosomal hexosaminidases (hHexA and hHexB, GH20) are mechanistically related enzymes; therefore, selective inhibition of these enzymes is crucial in terms of potential applications. In order to extend the structure-activity relationships of OGA inhibitors, a series of 2-acetamido-2-deoxy-d-glucono-1,5-lactone sulfonylhydrazones was prepared from d-glucosamine. The synthetic sequence involved condensation of N-acetyl-3,4,6-tri-O-acetyl-d-glucosamine with arenesulfonylhydrazines, followed by MnO2 oxidation to the corresponding glucono-1,5-lactone sulfonylhydrazones. Removal of the O-acetyl protecting groups by NH3/MeOH furnished the test compounds. Evaluation of these compounds by enzyme kinetic methods against hOGA and hHexB revealed potent nanomolar competitive inhibition of both enzymes, with no significant selectivity towards either. The most efficient inhibitor of hOGA was 2-acetamido-2-deoxy-d-glucono-1,5-lactone 1-naphthalenesulfonylhydrazone (5f, Ki = 27 nM). This compound had a Ki of 6.8 nM towards hHexB. To assess the binding mode of these inhibitors to hOGA, computational studies (Prime protein-ligand refinement and QM/MM optimizations) were performed, which suggested the binding preference of the glucono-1,5-lactone sulfonylhydrazones in an s-cis conformation for all test compounds.
- MeSH
- antigeny nádorové chemie metabolismus MeSH
- beta-hexosaminidasa, beta řetězec chemie metabolismus MeSH
- histonacetyltransferasy chemie metabolismus MeSH
- hyaluronoglukosaminidasa chemie metabolismus MeSH
- hydrazony chemická syntéza chemie farmakologie MeSH
- inhibitory enzymů chemická syntéza chemie farmakologie MeSH
- laktony chemie MeSH
- lidé MeSH
- molekulární konformace MeSH
- molekulární modely MeSH
- oxidy chemie MeSH
- sloučeniny manganu chemie MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Cobalt oxide nanoparticles were prepared via green chemistry route and fully characterized by Field Emission Scanning Electron Microscope (FESEM), Energy-dispersive X-ray spectroscopy (EDAX), X-ray diffraction (XRD), High-resolution transmission electron microscopy (HRTEM) and Transmission electron microscopy (TEM) analyses; the CoO and Co3O4 nanoparticles, in sheet-shaped cobalt oxide form, ensued simultaneously in one step. The varying concentrations of NPs were analyzed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test on the cancer cell line (U87) which revealed that with increasing concentration of cobalt oxide nanoparticles, the survival rate of U87 tumor cells decreases; IC50 of nanoparticles being ~ 55 µg/ml-1.
- MeSH
- antibakteriální látky chemie MeSH
- difrakce rentgenového záření MeSH
- inhibiční koncentrace 50 MeSH
- kobalt chemie MeSH
- koncentrace vodíkových iontů MeSH
- kovové nanočástice chemie MeSH
- lidé MeSH
- magnetismus MeSH
- mikrobiální testy citlivosti MeSH
- nádorové buněčné linie MeSH
- nanomedicína metody MeSH
- nanotechnologie metody MeSH
- oxidy chemie MeSH
- povrchově aktivní látky MeSH
- protinádorové látky farmakologie MeSH
- rostlinné extrakty MeSH
- rozmarýn MeSH
- rozpustnost MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- technologie zelené chemie metody MeSH
- teplota MeSH
- tetrazoliové soli chemie MeSH
- thiazoly chemie MeSH
- transmisní elektronová mikroskopie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The remediation of a soil contaminated with Zn, Pb and Cd was tested by using biochar (BC), nano zero-valent iron (nZVI) and a combination of these two (BC + nZVI). Each amendment was individually applied to the soil at 2 wt%. We tested the influence of (i) the used amendments, (ii) time, and (iii) soil moisture conditions on the metal availability and soil physico-chemical parameters using various extraction methods, as well as soil pore water samplings. We found that metal availability was mainly affected by pH under the influence of time and water content. Among the tested treatments, BC was the most successful, resulting in the lowest amounts of the target metals in the pore water and the smallest temporal changes in metal concentrations and pH in the soil. The use of nZVI efficiently decreased water-extractable Pb in the short- and long-term. The BC + nZVI treatment also yielded promising results regarding the immobilisation of the studied metals. Time provoked a general decrease in pH, which occasionally increased the available metal concentrations. Raising the soil water content increased the pH and subsequently lowered the available metal concentrations in the pore water. The mechanisms of metal stabilisation were further investigated by SEM/EDS. The results indicated that the used soil amendments enhanced the binding of Zn, Pb, and Cd on Fe/Mn/Al oxides/hydroxides, which in turn resulted in the stabilisation of the target metals.
- MeSH
- dřevěné a živočišné uhlí chemie MeSH
- kadmium analýza MeSH
- látky znečišťující půdu analýza chemie MeSH
- olovo MeSH
- oxidy chemie MeSH
- půda chemie MeSH
- regenerace a remediace životního prostředí metody MeSH
- těžké kovy analýza chemie MeSH
- železo chemie MeSH
- zinek analýza MeSH
- Publikační typ
- časopisecké články MeSH
The impact of four pre-treatment techniques on the surface morphology and chemistry, residual stress, mechanical properties, corrosion resistance in a physiological saline solution and cell colonization of commercially pure titanium is examined in detail. Mechanical polishing, electrochemical etching, chemical etching in Kroll's reagent, and ion sputter etching with argon ions were applied. Surface morphologies reflect the nature of surface layer removal. Significant roughening of the surface and a characteristic microtopology become apparent as a result of the sensitivity of chemical and ion sputter etching to the grain orientation. The hardness in the near surface region was controlled by the amount of residual stress. Etching of the stressed surface layer led to a reduction in residual stress and surface hardness. A compact passivation layer composed of TiO, TiO2 and Ti2O3 native oxides imparted high corrosion resistance to the surface after mechanical polishing, chemical and electrochemical etching. The ion sputter etched surface showed substantially reduced corrosion resistance, where the corrosion process was controlled by electron transfer. The specific topology affected the adhesion of the cell to the surface rather than the cell area coverage. The cell area coverage increased with the corrosion stability of the surface.
- MeSH
- buněčné linie MeSH
- elektrochemické techniky MeSH
- koroze MeSH
- lidé MeSH
- oxidy chemie MeSH
- povrchové vlastnosti MeSH
- testování materiálů MeSH
- titan chemie MeSH
- tvrdost MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Tungsten oxide-based bulk and nanocrystalline materials are widely used as photocatalytic and photo- and electrochromic materials, as well as materials for biomedical applications. In our work, we focused our attention on the effect of sodium cations on the structure and photochromic properties of the WO3@PVP aqueous sols. To establish the effect, the sols were synthesized by either simple pH adjusting of sodium or ammonium tungstates' solutions, or using an ion exchange technique to remove the cations from the materials to the greatest possible extent. We showed that the presence of sodium cations in WO3@PVP favors the formation of reduced tungsten species (W+5) upon UV irradiation of the materials, strongly affecting their photochromic and photocatalytic properties. The pronounced photoreductive properties of WO3@PVP sols in photocatalytic reactions were demonstrated. Due to photoreductive properties, photochromic sols of tungsten oxide can act as effective photoprotectors in photooxidation processes. We believe that our work provides a considerable contribution to the elucidation of photochromic and redox phenomena in WO3-based materials.
- MeSH
- difrakce rentgenového záření MeSH
- fotochemické procesy MeSH
- katalýza MeSH
- kationty MeSH
- koncentrace vodíkových iontů MeSH
- maloúhlový rozptyl MeSH
- molekulární struktura MeSH
- nanočástice chemie MeSH
- oxidy chemie MeSH
- povidon chemie MeSH
- sodík chemie MeSH
- ultrafialové záření MeSH
- velikost částic MeSH
- wolfram chemie MeSH
- Publikační typ
- časopisecké články MeSH
Combining the unique properties of peptides as versatile tools for nano- and biotechnology with lead halide perovskite nanoparticles can bring exceptional opportunities for the development of optoelectronics, photonics, and bioelectronics. As a first step towards this challenge sub 10 nm methylammonium lead bromide perovskite colloidal nanoparticles have been synthetizes using commercial cyclic peptide Cyclo(RGDFK), containing 5 amino acids, as a surface stabilizer. Perovskite nanoparticles passivated with Cyclo(RGDFK) possess charge transfer from the perovskite core to the peptide shell, resulting in lower photoluminescence quantum yields, which however opens a path for the application where charge transfer is favorable.
