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Development of a SCAR marker by inter-simple sequence repeat for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa KüHN

Gao L, Chen WQ, Liu TG.

. 2010 ; 55 (3) : 258-264.

Jazyk angličtina Země Česko

Perzistentní odkaz   https://www.medvik.cz/link/bmc10017815

Dwarf bunt of wheat, caused by Tilletia controversa KüHN: , is a destructive disease on wheat as well as an important internationally quarantined disease in many countries. The primer ISSR818 generated a polymorphic pattern displaying a 867-bp DNA fragment specific for T. controversa. The marker was converted into a sequence characterized amplified region (SCAR), and specific primers (TCKSF3/TCKSR3) designed for use in PCR detection assays; they amplified a unique DNA fragment in all isolates of T. controversa but not in the related pathogens. The detection limit with the primer set (TCKSF3/TCKSR3) was 5 ng of DNA which could be obtained from 5.5 microg of teliospores in a 25-microL PCR reaction mixture.

Bibliografie atd.

Lit.: 49

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