-
Je něco špatně v tomto záznamu ?
Kinetics of Toll-like receptor-4 splice variants expression in lipopolysaccharide-stimulated antigen presenting cells of healthy donors and patients with cystic fibrosis
I Jarešová, D. Rožková, R. Špíšek, A. Janda, J. Brázová, A. Šedivá
Jazyk angličtina Země Francie
Grantová podpora
NR8458
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Část
Zdroj
E-zdroje NLK Online
ScienceDirect (archiv) od 1999-01-01 do 2009-12-31Elsevier Open Access Journals od 1999-01-01 do Před 1 rokem
Odkazy
Knihovny.cz E-zdroje
- MeSH
- alternativní sestřih MeSH
- antigen prezentující buňky chemie imunologie MeSH
- cystická fibróza imunologie MeSH
- dendritické buňky imunologie MeSH
- dospělí MeSH
- financování organizované MeSH
- kultivované buňky MeSH
- lidé MeSH
- lipopolysacharidy imunologie MeSH
- messenger RNA analýza MeSH
- monocyty imunologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí metody MeSH
- regulace genové exprese MeSH
- stanovení celkové genové exprese MeSH
- TNF-alfa biosyntéza MeSH
- toll-like receptor 4 biosyntéza genetika MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
Toll-like receptors (TLR) are key components of innate immune system. As TLR activation could induce potentially harmful inflammatory response, activation of TLR signaling pathways has to be under tight control. Besides other control mechanisms, an inhibitory function of murine TLR4 splice variants was recently demonstrated. In this study we investigated expression of four TLR4 splice variants in human antigen presenting cells (APC). Furthermore, we studied modification in TLR4 splice variants expression in APC in cystic fibrosis (CF) patients chronically infected by Gram-negative bacteria. We developed a novel reliable real-time PCR detection system that allowed monitoring of individual TLR4 splice variants expression. In APC from healthy donors we detected a characteristic transient increase of two out of four splice variants after lipopolysaccharide (LPS) stimulation. Similarly to murine TLR4, one of these variants, NM 003266, might translate to a potentially inhibitory protein. In contrast to controls, CF monocytes had significantly changed LPS-induced expression of TLR4 gene and its variants including reduced ability to up-regulate the expression of the potentially inhibitory variant upon stimulation. In accordance with this observation, monocytes from CF patients produced significantly more tumor necrosis factor after LPS stimulation than healthy controls. Our results thus describe the kinetics of TLR4 splicing variants expression after LPS stimulation and indicate a possible alteration of its regulation in CF patients.
- 000
- 00000naa 2200000 a 4500
- 001
- bmc10026337
- 003
- CZ-PrNML
- 005
- 20130313090133.0
- 008
- 101018s2007 fr e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a fr
- 100 1_
- $a Zentsová-Jarešová, Irena $7 xx0102059
- 245 10
- $a Kinetics of Toll-like receptor-4 splice variants expression in lipopolysaccharide-stimulated antigen presenting cells of healthy donors and patients with cystic fibrosis / $c I Jarešová, D. Rožková, R. Špíšek, A. Janda, J. Brázová, A. Šedivá
- 314 __
- $a Institute of Immunology, University Hospital Motol and 2nd Medical School, Charles University, V Uvalu 84, 15006 Prague, Czech Republic.
- 520 9_
- $a Toll-like receptors (TLR) are key components of innate immune system. As TLR activation could induce potentially harmful inflammatory response, activation of TLR signaling pathways has to be under tight control. Besides other control mechanisms, an inhibitory function of murine TLR4 splice variants was recently demonstrated. In this study we investigated expression of four TLR4 splice variants in human antigen presenting cells (APC). Furthermore, we studied modification in TLR4 splice variants expression in APC in cystic fibrosis (CF) patients chronically infected by Gram-negative bacteria. We developed a novel reliable real-time PCR detection system that allowed monitoring of individual TLR4 splice variants expression. In APC from healthy donors we detected a characteristic transient increase of two out of four splice variants after lipopolysaccharide (LPS) stimulation. Similarly to murine TLR4, one of these variants, NM 003266, might translate to a potentially inhibitory protein. In contrast to controls, CF monocytes had significantly changed LPS-induced expression of TLR4 gene and its variants including reduced ability to up-regulate the expression of the potentially inhibitory variant upon stimulation. In accordance with this observation, monocytes from CF patients produced significantly more tumor necrosis factor after LPS stimulation than healthy controls. Our results thus describe the kinetics of TLR4 splicing variants expression after LPS stimulation and indicate a possible alteration of its regulation in CF patients.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a alternativní sestřih $7 D017398
- 650 _2
- $a antigen prezentující buňky $x chemie $x imunologie $7 D000938
- 650 _2
- $a kultivované buňky $7 D002478
- 650 _2
- $a cystická fibróza $x imunologie $7 D003550
- 650 _2
- $a dendritické buňky $x imunologie $7 D003713
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a stanovení celkové genové exprese $7 D020869
- 650 _2
- $a regulace genové exprese $7 D005786
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a lipopolysacharidy $x imunologie $7 D008070
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a monocyty $x imunologie $7 D009000
- 650 _2
- $a messenger RNA $x analýza $7 D012333
- 650 _2
- $a polymerázová řetězová reakce s reverzní transkripcí $x metody $7 D020133
- 650 _2
- $a toll-like receptor 4 $x biosyntéza $x genetika $7 D051197
- 650 _2
- $a TNF-alfa $x biosyntéza $7 D014409
- 650 _2
- $a financování organizované $7 D005381
- 700 1_
- $a Rožková, Daniela, $d 1979- $7 xx0118296
- 700 1_
- $a Špíšek, Radek, $d 1975- $7 nlk20030145288
- 700 1_
- $a Janda, Aleš, $d 1974- $7 mzk2007382260
- 700 1_
- $a Brázová, Jitka $7 xx0082231
- 700 1_
- $a Šedivá, Anna, $d 1955- $7 xx0000191
- 773 0_
- $w MED00006000 $t Microbes and infection $g Roč. 9, č. 11 (2007), s. 1359-1367 $x 1286-4579
- 910 __
- $a ABA008 $b x $y 7
- 990 __
- $a 20110112120804 $b ABA008
- 991 __
- $a 20130313090348 $b ABA008
- 999 __
- $a ok $b bmc $g 801442 $s 666189
- BAS __
- $a 3
- BMC __
- $a 2007 $b 9 $c 11 $d 1359-1367 $i 1286-4579 $m Microbes and infection $n Microbes Infect $x MED00006000
- GRA __
- $a NR8458 $p MZ0
- LZP __
- $a 2010-B/mk