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Multichannel SPR biosensor for detection of endocrine-disrupting compounds

J Dostalek, J Pribyl, J Homola, P Skladal

. 2007 ; 389 (6) : 1841-1847.

Jazyk angličtina Země Německo

Perzistentní odkaz   https://www.medvik.cz/link/bmc10026696
E-zdroje Online

NLK ProQuest Central od 2005-01-01 do 2008-12-31
Medline Complete (EBSCOhost) od 2003-01-01 do Před 1 rokem
Health & Medicine (ProQuest) od 2005-01-01 do 2008-12-31

A surface plasmon resonance (SPR) biosensor for simultaneous detection of multiple organic pollutants exhibiting endocrine-disrupting activity, namely atrazine, benzo[a]pyrene, 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-nonylphenol, is reported. The biosensor utilizes a multichannel SPR sensor based on wavelength modulation of SPR and wavelength division multiplexing (WDM) of sensing channels, antibodies as biorecognition element and a competitive immunoassay detection format. An analysis time of 45 min (including 30-min incubation of the sample with antibodies) and limits of detection as low as 0.05, 0.07, 0.16 and 0.26 ng mL(-1) are demonstrated for benzo[a]pyrene, atrazine, 2,4-D and 4-nonylphenol, respectively. The biosensor is also shown to be regenerable and suitable for repeated use.

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