A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.

Anotace

do not confuse with plasmon, extrachromosomal genetic material

DUI

D020349 MeSH Prohlížeč

CUI

M0029983

Předchozí užití

Biosensing Techniques (1991-1998)

Historická pozn.

1999

Veřejná pozn.

1999