Surface Plasmon Resonance [povrchová plasmonová rezonance]

topical
96
Terms

rezonance povrchového plazmonu
rezonance povrchových plasmonů
rezonanční excitace povrchových plazmonů
SPR

Persistent link   https://www.medvik.cz/link/D020349
Definition

A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.

Annotation
do not confuse with plasmon, extrachromosomal genetic material
DUI
D020349 MeSH Browser
CUI
M0029983
Previous indexing
Biosensing Techniques (1991-1998)
History note
1999
Public note
1999

Allowable subheadings

CL
classification 0
EC
economics 0
ES
ethics 0
HI
history 0
IS
instrumentation 22
MT
methods 42
ST
standards 0
SN
statistics & numerical data 2
TD
trends 1
VE
veterinary 0

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E05 Investigative Techniques 209
E05.196 Chemistry Techniques, Analytical 1 182
E05.196.039 Activation Analysis 55
E05.196.059 Analytic Sample Preparation Methods 87
E05.196.080 Binding, Competitive 61
E05.196.085 Biuret Reaction 2
E05.196.131 Calorimetry 147
E05.196.150 Chemical Precipitation 122
E05.196.155 Chemical Fractionation 76
E05.196.168 Chemometrics 1
E05.196.181 Chromatography 834
E05.196.278 Countercurrent Distribution 4
E05.196.300 Crystallization 186
E05.196.309 Crystallography 69
E05.196.335 Desiccation 51
E05.196.344 Deuterium Exchange Measurement 14
E05.196.353 Dialysis 772
E05.196.370 Differential Thermal Analysis 7
E05.196.385 Electroosmosis 11
E05.196.401 Electrophoresis 878
E05.196.427 Enzyme Assays 90
E05.196.454 Filtration 323
E05.196.460 Flow Injection Analysis 67
E05.196.500 Glucose Clamp Technique 81
E05.196.566 Mass Spectrometry 840
E05.196.620 Microchemistry 56
E05.196.655 Molecular Imprinting 26
E05.196.680 Nucleotide Mapping 14
E05.196.690 Osmometry 1
E05.196.700 Peptide Mapping 56
E05.196.712 Photometry 381
E05.196.749 Polarography 578
E05.196.778 Quartz Crystal Microbalance Techniques 11
E05.196.808 Refractometry 53
E05.196.822 Scattering, Radiation 49
E05.196.867 Spectrum Analysis 825
E05.196.885 Spin Trapping 12
E05.196.890 Surface Plasmon Resonance 96
E05.196.904 Thermogravimetry 24
E05.196.922 Titrimetry 150
E05.196.941 Ultracentrifugation 66
E05.601 Molecular Probe Techniques 38
E05.601.043 Biosensing Techniques 343
E05.601.043.700 Surface Plasmon Resonance 96

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