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High-resolution melt curve analysis: initial screening for mutations in BCR-ABL kinase domain
KM Polakova, T Lopotova, H Klamova, J Moravcova
Language English Country Great Britain
Document type Evaluation Study
Grant support
NR8758
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
ScienceDirect (archiv)
from 1993-01-01 to 2009-12-31
- MeSH
- Fusion Proteins, bcr-abl MeSH
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics MeSH
- Adult MeSH
- False Positive Reactions MeSH
- Financing, Government MeSH
- Middle Aged MeSH
- Humans MeSH
- Mutation MeSH
- DNA Mutational Analysis methods MeSH
- Protein-Tyrosine Kinases genetics MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Evaluation Study MeSH
Mutations in BCR-ABL kinase domain are associated with resistance to tyrosine kinase inhibitors in some patients with chronic myeloid leukemia. Therefore, mutation detection becomes essential in such patients. We aimed to apply high-resolution melt curve analysis (HRM) for a rapid screening prior to sequencing to select only mutation positive samples. One hundred and one samples with different mutations and mutational ratios were used for HRM testing. HRM results of 100/101 samples were concordant with sequencing data. We found HRM as a suitable and sensitive method for initial rapid screening of BCR-ABL KD mutations to sequence only positive samples.
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- $a Mutations in BCR-ABL kinase domain are associated with resistance to tyrosine kinase inhibitors in some patients with chronic myeloid leukemia. Therefore, mutation detection becomes essential in such patients. We aimed to apply high-resolution melt curve analysis (HRM) for a rapid screening prior to sequencing to select only mutation positive samples. One hundred and one samples with different mutations and mutational ratios were used for HRM testing. HRM results of 100/101 samples were concordant with sequencing data. We found HRM as a suitable and sensitive method for initial rapid screening of BCR-ABL KD mutations to sequence only positive samples.
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