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Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart

J. Kolářová, K. Fialová, O. Janoušek, M. Nováková, I. Provazník

. 2010 ; 59 (Suppl. 1) : S71-S80.
. 2010 ; 59 (Suppl. 1) : S71-S80.

Jazyk angličtina Země Česko

Perzistentní odkaz   https://www.medvik.cz/link/bmc11004400

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dualwavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.

Methods in Experimental and Clinical Cardiovascular Research. 37th Workshop of the Committee of Experimental Cardiology, Blansko, 2010

Bibliografie atd.

Lit.: 32

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