-
Je něco špatně v tomto záznamu ?
Pontin is localized in nucleolar fibrillar centers
Z. Cvackova, K.F. Albring, K. Koberna, A. Ligasova, O. Huber, I. Raska, D. Stanek
Jazyk angličtina Země Německo
Typ dokumentu práce podpořená grantem
NLK
ProQuest Central
od 1997-03-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2000-03-01 do 2015-12-31
Health & Medicine (ProQuest)
od 1997-03-01 do Před 1 rokem
- MeSH
- buněčné jadérko enzymologie ultrastruktura MeSH
- DNA-helikasy metabolismus MeSH
- genetická transkripce MeSH
- HeLa buňky MeSH
- lidé MeSH
- RNA ribozomální biosyntéza MeSH
- RNA-polymerasa I metabolismus MeSH
- transkripční iniciační komplex Pol1 - proteiny metabolismus MeSH
- transmisní elektronová mikroskopie MeSH
- transportní proteiny metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Pontin is a multifunctional protein having roles in various cellular processes including regulation of gene expression. Here, we addressed Pontin intracellular localization using two different monoclonal antibodies directed against different Pontin epitopes. For the first time, Pontin was directly visualized in nucleoli where it co-localizes with Upstream Binding Factor and RNA polymerase I. Nucleolar localization of Pontin was confirmed by its detection in nucleolar extracts and by electron microscopy, which revealed Pontin accumulation specifically in the nucleolar fibrillar centers. Pontin localization in the nucleolus was dynamic and Pontin accumulated in large nucleolar dots mainly during S-phase. Pontin concentration in the large nucleolar dots correlated with reduced transcriptional activity of nucleoli. In addition, Pontin was found to associate with RNA polymerase I and to interact in a complex with c-Myc with rDNA sequences indicating that Pontin is involved in the c-Myc-dependent regulation of rRNA synthesis.
Citace poskytuje Crossref.org
- 000
- 02580naa 2200409 a 4500
- 001
- bmc11006396
- 003
- CZ-PrNML
- 005
- 20121031160433.0
- 008
- 110401s2008 gw e eng||
- 009
- AR
- 024 __
- $a 10.1007/s00412-008-0170-8 $2 doi
- 035 __
- $a (PubMed)18548265
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Cvačková, Zuzana $7 xx0106591
- 245 10
- $a Pontin is localized in nucleolar fibrillar centers / $c Z. Cvackova, K.F. Albring, K. Koberna, A. Ligasova, O. Huber, I. Raska, D. Stanek
- 314 __
- $a Institute of Cellular Biology and Pathology, 1st Faculty of Medicine, Charles University in Prague, Albertov 4, 128 00 Prague 2, Czech Republic.
- 520 9_
- $a Pontin is a multifunctional protein having roles in various cellular processes including regulation of gene expression. Here, we addressed Pontin intracellular localization using two different monoclonal antibodies directed against different Pontin epitopes. For the first time, Pontin was directly visualized in nucleoli where it co-localizes with Upstream Binding Factor and RNA polymerase I. Nucleolar localization of Pontin was confirmed by its detection in nucleolar extracts and by electron microscopy, which revealed Pontin accumulation specifically in the nucleolar fibrillar centers. Pontin localization in the nucleolus was dynamic and Pontin accumulated in large nucleolar dots mainly during S-phase. Pontin concentration in the large nucleolar dots correlated with reduced transcriptional activity of nucleoli. In addition, Pontin was found to associate with RNA polymerase I and to interact in a complex with c-Myc with rDNA sequences indicating that Pontin is involved in the c-Myc-dependent regulation of rRNA synthesis.
- 590 __
- $a bohemika - dle Pubmed
- 650 _2
- $a transportní proteiny $x metabolismus $7 D002352
- 650 _2
- $a buněčné jadérko $x enzymologie $x ultrastruktura $7 D002466
- 650 _2
- $a DNA-helikasy $x metabolismus $7 D004265
- 650 _2
- $a HeLa buňky $7 D006367
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a transmisní elektronová mikroskopie $7 D046529
- 650 _2
- $a transkripční iniciační komplex Pol1 - proteiny $x metabolismus $7 D035463
- 650 _2
- $a RNA-polymerasa I $x metabolismus $7 D012318
- 650 _2
- $a RNA ribozomální $x biosyntéza $7 D012335
- 650 _2
- $a genetická transkripce $7 D014158
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Albring, Kai,F $7 gn_A_00003588
- 700 1_
- $a Koberna, Karel, $d 1965- $7 xx0076209
- 700 1#
- $a Ligasová, Anna. $7 _AN056303
- 700 1_
- $a Huber, Otmar
- 700 1_
- $a Raška, Ivan, $d 1945- $7 nlk19990073764
- 700 1_
- $a Staněk, David $7 xx0140539
- 773 0_
- $t Chromosoma $w MED00002160 $g Roč. 117, č. 5 (2008), s. 487-497 $x 0009-5915
- 910 __
- $a ABA008 $b x $y 2
- 990 __
- $a 20110414102936 $b ABA008
- 991 __
- $a 20121031160437 $b ABA008
- 999 __
- $a ok $b bmc $g 833990 $s 698497
- BAS __
- $a 3
- BMC __
- $a 2008 $b 117 $c 5 $d 487-497 $m Chromosoma $n Chromosoma $x MED00002160 $i 0009-5915
- LZP __
- $a 2011-1B09/jjme
