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Determination of ammonia, creatinine and inorganic cations in urine using CE with contactless conductivity detection
P Tuma, E Samcova, F Duska
Language English Country Germany
- MeSH
- Ammonia urine MeSH
- Electric Conductivity MeSH
- Electrophoresis, Capillary methods statistics & numerical data MeSH
- Financing, Organized MeSH
- Cations urine MeSH
- Creatinine MeSH
- Humans MeSH
- Polyethylene Glycols MeSH
- Reproducibility of Results MeSH
- Solutions MeSH
- Check Tag
- Humans MeSH
CE with capacitively coupled contactless conductivity detection (C(4)D) was used to determine waste products of the nitrogen metabolism (ammonia and creatinine) and of biogenic inorganic cations in samples of human urine. The CE separation was performed in two BGEs, consisting of 2 M acetic acid + 1.5 mM crown ether 18-crown-6 (BGE I) and 2 M acetic acid + 2% w/v PEG (BGE II). Only BGE II permitted complete separation of all the analytes in a model sample and in real urine samples. The LOD values for the optimized procedure ranged from 0.8 microM for Ca(2+) and Mg(2+) to 2.9 microM for NH(4)(+) (in terms of mass concentration units, from 7 microg/L for Li(+) to 102 microg/L for creatinine). These values are adequate for determination of NH(4)(+), creatinine, Na(+), K(+), Ca(2+) and Mg(2+) in real urine samples.
References provided by Crossref.org
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- 02213naa 2200349 a 4500
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- bmc11006681
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- CZ-PrNML
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- 20121031103402.0
- 008
- 110405s2008 gw e eng||
- 009
- AR
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- $a 10.1002/jssc.200700655 $2 doi
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- $a (PubMed)18563755
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- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
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- $a eng
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- $a Tůma, Petr $7 xx0105786
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- $a Determination of ammonia, creatinine and inorganic cations in urine using CE with contactless conductivity detection / $c P Tuma, E Samcova, F Duska
- 314 __
- $a Institute of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic. petr.tuma@lf3.cuni.cz
- 520 9_
- $a CE with capacitively coupled contactless conductivity detection (C(4)D) was used to determine waste products of the nitrogen metabolism (ammonia and creatinine) and of biogenic inorganic cations in samples of human urine. The CE separation was performed in two BGEs, consisting of 2 M acetic acid + 1.5 mM crown ether 18-crown-6 (BGE I) and 2 M acetic acid + 2% w/v PEG (BGE II). Only BGE II permitted complete separation of all the analytes in a model sample and in real urine samples. The LOD values for the optimized procedure ranged from 0.8 microM for Ca(2+) and Mg(2+) to 2.9 microM for NH(4)(+) (in terms of mass concentration units, from 7 microg/L for Li(+) to 102 microg/L for creatinine). These values are adequate for determination of NH(4)(+), creatinine, Na(+), K(+), Ca(2+) and Mg(2+) in real urine samples.
- 650 _2
- $a amoniak $x moč $7 D000641
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- $a kationty $x moč $7 D002412
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- $a kreatinin $7 D003404
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- $a Duška, František, $d 1976- $7 mzk2007386027
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- $t Journal of Separation Science $w MED00006463 $g Roč. 31, č. 12 (2008), s. 2260-2264
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- $a 2011-4B/ewme
