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Molecular detection of Borrelia bissettii DNA in serum samples from patients in the Czech Republic with suspected borreliosis
N. Rudenko, M. Golovchenko, D. Růžek, N. Piskunova, N. Mallátová, L. Grubhoffer
Jazyk angličtina Země Velká Británie
NLK
ProQuest Central
od 1996-01-01 do 2012-12-31
Medline Complete (EBSCOhost)
od 2006-01-01 do 2014-12-15
Health & Medicine (ProQuest)
od 1996-01-01 do 2012-12-31
Wiley Online Library (archiv)
od 1997-01-01 do 2012-12-31
Public Health Database (ProQuest)
od 1996-01-01 do 2012-12-31
- MeSH
- bakteriální RNA genetika MeSH
- Borrelia genetika izolace a purifikace MeSH
- DNA bakterií genetika chemie krev MeSH
- financování organizované MeSH
- fylogeneze MeSH
- geny rRNA MeSH
- infekce bakteriemi rodu Borrelia diagnóza MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce metody MeSH
- ribozomální DNA genetika chemie MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie nukleových kyselin MeSH
- sérum mikrobiologie MeSH
- Check Tag
- lidé MeSH
- Geografické názvy
- Česká republika MeSH
Until recently, three spirochete genospecies were considered to be the causative agents of Lyme borreliosis (LB) in Europe: Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii. However, the DNA of Borrelia valaisiana, Borrelia lusitaniae, Borrelia spielmanii and Borrelia bissettii has already been detected in samples of human origin, or the spirochetes were isolated from the patients with symptoms of LB. Molecular analysis of 12 selected serum samples collected in the regional hospital confirmed the presence of B. bissettii DNA in cases of single and multiple infection in patients with symptomatic borreliosis or chronic borrelial infection. The presence of B. bissettii as a single strain in patients provides strong support of the fact that B. bissettii might be a causative agent of the disease. After the first isolation of B. bissettii from the samples of human origin in Slovenia, following the detection of this species in cardiac valve tissue of the patient with endocarditis and aortic valve stenosis in the Czech Republic, here we present additional molecular data supporting the involvement of B. bissettii in LB in Europe.
Citace poskytuje Crossref.org
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