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Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres
J. Kahánková, A. Španová, R. Pantůček, D. Horák, J. Doškař, B. Rittich
Jazyk angličtina Země Nizozemsko
- MeSH
- DNA virů genetika izolace a purifikace MeSH
- extrakce na pevné fázi metody MeSH
- financování organizované MeSH
- genetické techniky MeSH
- magnetismus MeSH
- mikrosféry MeSH
- polymerázová řetězová reakce MeSH
- stafylokokové bakteriofágy genetika izolace a purifikace MeSH
- Staphylococcus aureus virologie MeSH
Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microl) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection.
Citace poskytuje Crossref.org
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- $a Masaryk University, Faculty of Science, Institute of Experimental Biology, Tvrdeho 14, CZ-602 00 Brno, Czech Republic.
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- $a Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microl) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection.
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