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Reduced inducibility of SOCS3 by interferon gamma associates with higher resistance of human breast cancer lines as compared to normal mammary epithelial cells
K. Součková, A. Kovařík, L. Dušek, L. Humpolíková-Adámková, L. Lauerová, E. Krejčí, E. Matoušková, E. Buršíková, M. Fojtová, J. Kovařík
Language English Country Slovakia
Document type Comparative Study, Research Support, Non-U.S. Gov't
- MeSH
- Drug Resistance, Neoplasm MeSH
- Phosphorylation MeSH
- Interferon-gamma pharmacology MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Breast Neoplasms drug therapy metabolism pathology MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Suppressor of Cytokine Signaling Proteins genetics MeSH
- Breast metabolism drug effects MeSH
- STAT1 Transcription Factor metabolism MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
The resistance to interferons (IFNs) limits their anticancer therapeutic efficacy. Here we studied the antiproliferative effect of interferon gamma in relation to SOCS3 expression in a panel of breast cancer cell lines and normal mammary epithelial cells. Compared to normal cells most breast cancer lines (7/8) were highly resistant to IFN-gamma. Using Northern blot and real time RT-PCR we investigated transcription of SOCS3 genes. All normal epithelial cells (4/4) showed SOCS3 induction (2-14 fold) while most breast cancer lines did not or weakly activated SOCS3 after the interferon gamma treatment. Among the cancer lines, the MDA-MB-468 cells showed increased sensitivity to IFN-gamma and relatively high level of SOCS3 induction (2-3 fold). Together, there was a good correlation
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