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The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity
J. Králová, L. Račková, M. Pekarová, L. Kubala, R. Nosáľ, V. Jančinová, M. Číž, A. Lojek
Jazyk angličtina Země Nizozemsko
Typ dokumentu práce podpořená grantem
- MeSH
- buněčné extrakty MeSH
- buněčné linie MeSH
- down regulace MeSH
- dusitany metabolismus MeSH
- ethylendiaminy MeSH
- lipidy chemie MeSH
- lipopolysacharidy metabolismus MeSH
- makrofágy enzymologie patologie účinky léků MeSH
- myši MeSH
- nesedativní H1-antihistaminika farmakologie chemie MeSH
- oxid dusnatý metabolismus MeSH
- sulfanilamidy MeSH
- synthasa oxidu dusnatého, typ II metabolismus MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
H1-antihistamines are known to be important modulators of inflammatory response. However, the information about the influence of these drugs on reactive nitrogen species generation is still controversial. The main aim of the present study was to investigate the effects of selected H1-antihistamines on nitric oxide production by lipopolysaccharide-stimulated murine macrophages RAW 264.7, measured as changes in inducible nitric oxide synthase (iNOS) protein expression in cell lysates by Western blotting and nitrite formation in cell supernatants using the Griess reaction. In pharmacological non-toxic concentrations, H1-antihistamines significantly inhibited nitrite accumulation that was not caused by the scavenging ability of drugs against nitric oxide, measured amperometrically. The degree of inhibition of nitrite accumulation positively correlated with the degree of tested lipophilicity, measured by reversed-phase thin layer chromatography. Furthermore, H1-antihistamines differentially modulated the iNOS protein expression. In conclusion, as was shown in this study, the modulation of nitric oxide production could be caused by the downregulation of iNOS protein expression and/or the iNOS protein activity.
Citace poskytuje Crossref.org
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- $a H1-antihistamines are known to be important modulators of inflammatory response. However, the information about the influence of these drugs on reactive nitrogen species generation is still controversial. The main aim of the present study was to investigate the effects of selected H1-antihistamines on nitric oxide production by lipopolysaccharide-stimulated murine macrophages RAW 264.7, measured as changes in inducible nitric oxide synthase (iNOS) protein expression in cell lysates by Western blotting and nitrite formation in cell supernatants using the Griess reaction. In pharmacological non-toxic concentrations, H1-antihistamines significantly inhibited nitrite accumulation that was not caused by the scavenging ability of drugs against nitric oxide, measured amperometrically. The degree of inhibition of nitrite accumulation positively correlated with the degree of tested lipophilicity, measured by reversed-phase thin layer chromatography. Furthermore, H1-antihistamines differentially modulated the iNOS protein expression. In conclusion, as was shown in this study, the modulation of nitric oxide production could be caused by the downregulation of iNOS protein expression and/or the iNOS protein activity.
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