• Je něco špatně v tomto záznamu ?

Newly designed, simple relief phase contrast for microscopy of microorganisms

Z. Žižka

. 2010 ; 55 (6) : 662-665.

Jazyk angličtina Země Česko

Perzistentní odkaz   https://www.medvik.cz/link/bmc12004007

A new method providing a relief phase contrast for investigation of microorganisms by optical microscopy used a neutral filter Zeiss NG 10/1 that could be controllably slid at a certain azimuthal angle below the aperture condenser diaphragm of the microscope phase contrast. Two ways of application are described depending on the type of the microscope: (1) in a special holder, and (2) fixed on a rubber ring. The device enabled us to obtain excellent results in the area of both optical microscopy and microphotography. With the microorganisms visualized, a better resolution, higher contrast and a significant 3D effect were obtained; outer morphology and organelles (chloroplasts, nuclei, granules, oil reserve vacuoles, etc.) could also be investigated.

Bibliografie atd.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc12004007
003      
CZ-PrNML
005      
20120209124246.0
007      
ta
008      
120208s2010 xr o f 000 0eng||
009      
AR
024    7_
$2 doi $a 10.1007/s12223-010-0107-5
040    __
$a ABA008 $d ABA008 $e AACR2 $b cze
041    0_
$a eng
044    __
$a xr
100    1_
$a Žižka, Zdeněk $7 xx0117543 $u Institute of Microbiology of the ASCR, v.v.i., 14220 Praha
245    10
$a Newly designed, simple relief phase contrast for microscopy of microorganisms / $c Z. Žižka
504    __
$a Literatura
520    9_
$a A new method providing a relief phase contrast for investigation of microorganisms by optical microscopy used a neutral filter Zeiss NG 10/1 that could be controllably slid at a certain azimuthal angle below the aperture condenser diaphragm of the microscope phase contrast. Two ways of application are described depending on the type of the microscope: (1) in a special holder, and (2) fixed on a rubber ring. The device enabled us to obtain excellent results in the area of both optical microscopy and microphotography. With the microorganisms visualized, a better resolution, higher contrast and a significant 3D effect were obtained; outer morphology and organelles (chloroplasts, nuclei, granules, oil reserve vacuoles, etc.) could also be investigated.
650    _2
$a financování organizované $7 D005381
650    _2
$a eukaryotické buňky $x cytologie $7 D005057
650    _2
$a mikrobiologické techniky $x metody $7 D008828
650    _2
$a mikroskopie fázově kontrastní $x metody $7 D008858
650    _2
$a organely $x ultrastruktura $7 D015388
650    _2
$a prokaryotické buňky $x cytologie $7 D011387
773    0_
$t Folia microbiologica $x 0015-5632 $g Roč. 55, č. 6 (2010), s. 662-665 $w MED00011005
910    __
$a ABA008 $b A 2403 $c 561 $y 2
990    __
$a 20120207143748 $b ABA008
991    __
$a 20120209124228 $b ABA008
999    __
$a ok $b bmc $g 896906 $s 760786
BAS    __
$a 3
BMC    __
$a 2010 $b 55 $c 6 $d 662-665 $i 0015-5632 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
LZP    __
$a 2012-03/mkme

Najít záznam

Citační ukazatele

Možnosti archivace