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Identification of Plesiomonas spp.: serological and MALDI-TOF MS methods

R. Kolínská, M. Dřevínek, E. Aldová, H. Žemličková

. 2010 ; 55 (6) : 669.672.

Jazyk angličtina Země Česko

Perzistentní odkaz   https://www.medvik.cz/link/bmc12004018

Biochemical and serological profiles of isolates of Plesiomonas shigelloides were assayed using standard procedures in isolates from various clinical samples. Seventy-four isolates, including P. shigelloides type strain, were further characterized by MALDI-TOF MS using 3-methoxy-4-hydroxycinnamic acid as matrix. Multiple ions in the 3- to 12-kDa mass range were found in the spectra of each strain, from which the "species-identifying" unique biomarker ions were identified. After creating the species-specific patterns, a spectral database was generated for reliable, rapid, reproducible and accurate identification of Plesiomonas strains. The classical strain description (biochemical and serological) was thus complemented with the metabolic (proteomic) characterization.

Citace poskytuje Crossref.org

Bibliografie atd.

Literatura

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