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Využití multiplexové analýzy ke sledování cytokinového spektra alergických pacientů [Multiplex analysis of cytokine production in allergic patients]

Alexandra Lochmanová, Jaromír Bystroň, Ivo Lochman

. 2008 ; 18 (1) : 4-9.

Language Czech Country Slovakia

Persistent link   https://www.medvik.cz/link/bmc12008094

The Th1/Th2 dysbalance plays an important role in the pathogenesis of human allergy.. The study was performed on 18 allergic patients, 5 healthy patients without any clinical history served as negative controls. Characterization of T cell response based on cytokine production was studied in whole blood ex vivo stimulation. IL-2, IL-4, IL-5, IL-10, TNF-alpha and IFN- cytokine response both spontaneous and to mitogens PHA and ConA was done by multiplexed ALBIA (Adressable Laser Bead Immunoaasays) with CBA Human Th1/Th2 Cytokine Kit (BD Bioscience). Compared with controls patients suffering from allergy had higher spontaneous production of IL 4 and higher TNFalpha and IL 10 production both spontaneous and after PHA and ConA stimulation. Multiplex technique becomes more complex and can quantify and detect dynamic changes in cytokine profiles. High effectivity and a good price make the method acceptable for cytokine assessment in laboratory routine.

Multiplex analysis of cytokine production in allergic patients

Bibliography, etc.

Literatura

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$a The Th1/Th2 dysbalance plays an important role in the pathogenesis of human allergy.. The study was performed on 18 allergic patients, 5 healthy patients without any clinical history served as negative controls. Characterization of T cell response based on cytokine production was studied in whole blood ex vivo stimulation. IL-2, IL-4, IL-5, IL-10, TNF-alpha and IFN- cytokine response both spontaneous and to mitogens PHA and ConA was done by multiplexed ALBIA (Adressable Laser Bead Immunoaasays) with CBA Human Th1/Th2 Cytokine Kit (BD Bioscience). Compared with controls patients suffering from allergy had higher spontaneous production of IL 4 and higher TNFalpha and IL 10 production both spontaneous and after PHA and ConA stimulation. Multiplex technique becomes more complex and can quantify and detect dynamic changes in cytokine profiles. High effectivity and a good price make the method acceptable for cytokine assessment in laboratory routine.
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