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Time-dependent changes in concentration of two clinically used acetylcholinesterase reactivators (HI-6 and obidoxime) in rat plasma determined by HPLC techniques after in vivo administration
Karasova J Zdarova, L Novotny, K Antos, H Zivna, K Kuca
Jazyk angličtina Země Japonsko
Typ dokumentu práce podpořená grantem
NLK
Free Medical Journals
od 1985
J-STAGE (Japan Science & Technology Information Aggregator, Electronic) - English
od 1985 do 2021
J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - English
od 1985 do 2021
Open Access Digital Library
od 1985-01-01
- MeSH
- indikátory a reagencie MeSH
- injekce intramuskulární MeSH
- kalibrace MeSH
- krevní proteiny chemie MeSH
- krysa rodu rattus MeSH
- kyselina trichloroctová chemie MeSH
- obidoxim chlorid farmakokinetika krev MeSH
- oximy farmakokinetika krev MeSH
- potkani Wistar MeSH
- pyridinové sloučeniny farmakokinetika krev MeSH
- reaktivátory cholinesterázy farmakokinetika krev MeSH
- reprodukovatelnost výsledků MeSH
- spektrofotometrie ultrafialová MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
A simple and reliable HPLC method for determination of rat plasma levels of clinically used acetylcholinesterase (AChE) reactivators (HI-6 and obidoxime) is presented in our study. Separation was carried out by HPLC using an octadecyl silica stationary phase and a mobile phase consisting of 24% acetonitrile and containing 5 mM sodium octanesulfonate and 5 mM tetramethylammonium chloride (pH 2.3). Following intramuscular administration of equimolar doses of both oximes (22.23 mg/kg), the maximum of HI-6 concentration in rat plasma was reached in about 20 min giving 15.26 +/- 1.71 microg/mL. The distribution of obidoxime was fast; the single maximum 23.62 +/- 3.563 microg/mL was recorded at about 10 min. HPLC with UV detection presented in our study is a general method which could be applied for quick measurements of bisquaternary AChE reactivators in rat plasma.
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- $a Žďárová Karasová, Jana, $d 1982- $7 xx0099787 $u Department of Toxicology, Faculty of Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic. karasova@pmfhk.cz
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- $a Time-dependent changes in concentration of two clinically used acetylcholinesterase reactivators (HI-6 and obidoxime) in rat plasma determined by HPLC techniques after in vivo administration / $c Karasova J Zdarova, L Novotny, K Antos, H Zivna, K Kuca
- 520 9_
- $a A simple and reliable HPLC method for determination of rat plasma levels of clinically used acetylcholinesterase (AChE) reactivators (HI-6 and obidoxime) is presented in our study. Separation was carried out by HPLC using an octadecyl silica stationary phase and a mobile phase consisting of 24% acetonitrile and containing 5 mM sodium octanesulfonate and 5 mM tetramethylammonium chloride (pH 2.3). Following intramuscular administration of equimolar doses of both oximes (22.23 mg/kg), the maximum of HI-6 concentration in rat plasma was reached in about 20 min giving 15.26 +/- 1.71 microg/mL. The distribution of obidoxime was fast; the single maximum 23.62 +/- 3.563 microg/mL was recorded at about 10 min. HPLC with UV detection presented in our study is a general method which could be applied for quick measurements of bisquaternary AChE reactivators in rat plasma.
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