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Wound healing gene therapy: cartilage regeneration induced by vascular endothelial growth factor plasmid
K. Kolostova, O. Taltynov, D. Pinterova, M. Boubelik, O. Raska, P. Hozak, M. Jirkovska, V. Bobek
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Analysis of Variance MeSH
- Cartilage injuries MeSH
- Neovascularization, Physiologic drug effects MeSH
- Wound Healing drug effects MeSH
- Immunohistochemistry MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Disease Models, Animal MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Plasmids MeSH
- Regeneration MeSH
- Feasibility Studies MeSH
- Vascular Endothelial Growth Factor A pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
AIMS: The identification of growth factors and cytokines with angiogenic activity has enabled new therapeutic treatments for a variety of diseases; this concept is called therapeutic angiogenesis. The vascular endothelial growth factor (VEGF) is the most critical regulator of vascular formation. In the present study, we were interested in the therapeutic angiogenesis effect using plasmid transfer of human complementary DNA VEGF(165) (phVEGF(165)) in experimental skin and cartilage trauma. METHODS: Ten BALB/c mice were used for cartilage injury model. At 6 weeks of age, all mice were ear-punched, resulting in 2-mm-diameter puncture through the center of both pinnae. Each mouse got phVEGF(165) injection into the first ear and vector without insert or saline injection into the second one. The healing process was followed. The hollow diameter was measured on days 0, 14, and 42. Histological sections of experimental and control pinnae were taken from days 1, 3, 5, 7, 9, 11, 13, 15, 20, and 30 after experimental injury for hematoxylin and eosin and periodic acid Schiff staining and for human VEGF immunocytochemistry. The expression of human VEGF was also checked by real-time polymerase chain reaction in formalin-fixed, paraffin-embedded tissue sections. KEY FINDINGS: In BALB/c mouse strain, a significant angiogenesis promotion and cartilage repair were observed after phVEGF(165) injection into the punched ear area. SIGNIFICANCE: We suggest that administering phVEGF(165) leads to faster cartilage regeneration even if not only on the angiogenic basis.
Department of Thoracic Surgery Medical Academy Wroclaw Poland
Department of Tumor Biology 3rd Faculty of Medicine Charles University Prague Czech Republic
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